Document Detail


Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3.
MedLine Citation:
PMID:  16914499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SPL3, SPL4 and SPL5 (SPL3/4/5) are closely related members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE family of transcription factors in Arabidopsis, and have a target site for the microRNA miR156 in their 3' UTR. The phenotype of Arabidopsis plants constitutively expressing miR156-sensitive and miR156-insensitive forms of SPL3/4/5 revealed that all three genes promote vegetative phase change and flowering, and are strongly repressed by miR156. Constitutive expression of miR156a prolonged the expression of juvenile vegetative traits and delayed flowering. This phenotype was largely corrected by constitutive expression of a miR156-insensitive form of SPL3. The juvenile-to-adult transition is accompanied by a decrease in the level of miR156 and an increase in the abundance of SPL3 mRNA. The complementary effect of hasty on the miR156 and SPL3 transcripts, as well as the miR156-dependent temporal expression pattern of a 35S::GUS-SPL3 transgene, suggest that the decrease in miR156 is responsible for the increase in SPL3 expression during this transition. SPL3 mRNA is elevated by mutations in ZIPPY/AGO7, RNA DEPENDENT RNA POLYMERASE 6 (RDR6) and SUPPRESSOR OF GENE SILENCING 3 (SGS3), indicating that it is directly or indirectly regulated by RNAi. However, our results indicate that RNAi does not contribute to the temporal expression pattern of this gene. We conclude that vegetative phase change in Arabidopsis is regulated by an increase in the expression of SPL3 and probably also SPL4 and SPL5, and that this increase is a consequence of a decrease in the level of miR156.
Authors:
Gang Wu; R Scott Poethig
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-08-16
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  133     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-28     Completed Date:  2007-01-04     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3539-47     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics*,  growth & development,  metabolism
Arabidopsis Proteins / genetics*,  metabolism,  physiology
DNA-Binding Proteins / genetics*,  metabolism,  physiology
Flowers / genetics,  growth & development,  metabolism
Gene Expression Regulation, Plant / genetics*
MicroRNAs / genetics*,  metabolism
Models, Genetic
Mutation / genetics
Phenotype
Plant Leaves / genetics,  growth & development,  metabolism
Time Factors
Transcription Factors / genetics*,  metabolism,  physiology
Grant Support
ID/Acronym/Agency:
R01 GM051893-08A1/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA-Binding Proteins; 0/MicroRNAs; 0/SPL3 protein, Arabidopsis; 0/SPL4 protein, Arabidopsis; 0/Transcription Factors; 0/ZIP protein, Arabidopsis
Comments/Corrections

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