Document Detail


Temporal and hormonal control of beta-1,3-glucanase in Phaseolus vulgaris L.
MedLine Citation:
PMID:  5427109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endo-beta-1, 3-glucanase (beta-1, 3-glucan 3-glucanhydrolase, EC 3.2.1.6) extracted from Phaseolus vulgaris L. cv. Red Kidney had a pH optimum of 5 and a temperature optimum of 50 C. Excision of plant tissue resulted in an increase in beta-1, 3-glucanase activity after a 6-hour lag period. The increase could be prevented by indole-3-acetic acid, gibberellic acid, and cytokinins. Ethylene (half-maximal concentration = 0.1 microliter/liter) promoted the synthesis of beta-1, 3-glucanase, and 10% CO(2) overcame some of the ethylene effect. Cycloheximide prevented the induction of beta-1, 3-glucanase, but actinomycin D and chromomycin A(3) had only a partial effect. The amount of callose in sieve tube cells correlated with levels of beta-1, 3-glucanase, suggesting that this enzyme played a role in the degradation of beta-1, 3-glucans.
Authors:
F B Abeles; L E Forrence
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  45     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1970 Apr 
Date Detail:
Created Date:  1970-08-26     Completed Date:  1970-08-26     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / pharmacology
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Ethylenes / pharmacology
Glycoside Hydrolases / metabolism*
Plant Growth Regulators / pharmacology
Plants / enzymology*
Polysaccharides / metabolism
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Ethylenes; 0/Plant Growth Regulators; 0/Polysaccharides; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; EC 3.2.1.-/Glycoside Hydrolases
Comments/Corrections

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