Document Detail


Temporal expression of cyclooxygenase-2 in peritoneal macrophages of rats and effects of panax notoginseng saponins.
MedLine Citation:
PMID:  19184357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To explore the temporal expression pattern of cyclooxygenase (COX)-2 and effects of panax notogensing saponins (PNS) in peritoneal macrophages of rats. MATERIALS AND METHODS: Phagocytosis function of peritoneal macrophages was measured by chicken red blood cell phagocytosis assay in vitro. Expression of COX-2 mRNA and protein, PGE(2) and PGD(2) production were determined with real-time PCR, Western blotting and radioimmunoassay, respectively. RESULTS: Phagocytosis function of macrophages increased significantly after stimulation and reached peak during 2-3h. Expression of COX-2 mRNA and its protein increased markedly after stimulation and reached the first peak at 2 h and 3h, respectively; and then decreased to reach a minimum at 24h. The second peak appeared at 36h. PNS (50, 100, 200 mg/kg) increased the phagocytosis function obviously at 2h, decreased the expression level of COX-2 and PGE(2) production at 2 h and elevated COX-2 expression and PGD(2) production at 36 h, respectively. CONCLUSION: COX-2 expression in peritoneal macrophages has a double-hump feature after stimulation. PNS enhanced phagocytosis, inhibiting COX-2 expression at an early stage and elevating it at a later stage.
Authors:
Z B Yuan; H G Zhang; Y Jia; Y Q Cheng; X H Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  58     ISSN:  1420-908X     ISO Abbreviation:  Inflamm. Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-04-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  74-80     Citation Subset:  IM    
Affiliation:
Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, Chongqing, China. zhibinyuan@sina.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickens
Cyclooxygenase 2 / genetics,  metabolism*
Dinoprostone / metabolism
Macrophages, Peritoneal* / drug effects,  enzymology
Male
Panax notoginseng / chemistry*
Prostaglandin D2 / metabolism
Random Allocation
Rats
Rats, Sprague-Dawley
Saponins / pharmacology*
Zymosan / pharmacology
Chemical
Reg. No./Substance:
0/Saponins; 363-24-6/Dinoprostone; 41598-07-6/Prostaglandin D2; 9010-72-4/Zymosan; EC 1.14.99.1/Cyclooxygenase 2

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