Document Detail


Temporal coincidence of the appearance of elongated spermatids and of histochemical reaction of 11 beta-hydroxysteroid dehydrogenase in rat Leydig cells.
MedLine Citation:
PMID:  8250289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The serial semithin sections of the testes of perfused rats (age: 25-41 postnatal days) were evaluated microscopically on each alternative day for the morphology of spermatogenetic cells and especially for spermatid differentiation. The histochemical reaction for non-specific esterases, 3 alpha-hydroxysteroid-dehydrogenase (HSDH), 3 beta-HSDH, 11 beta-HSDH, 17 beta-HSDH were performed in cryostat sections of corresponding rat testes (age: 25-55 days). Eight different types of germ cell associations (designated as A-H) appeared from day 25 to day 41. Seven steps of differentiation of spermatids were characterized. The turning point of spermatid maturation was observed on day 35. Prior to this day, mainly spermatids step 1 were identified. On day 35 spermatids steps 1-5 were present. The reactions of non-specific esterases, 3 beta-HSDH, 17 beta-HSDH and 3 alpha-HSDH were observed in the Leydig cells of all testes. Only from day 35 onwards could 11 beta-HSDH be demonstrated. The temporal sequence of the differentiation of spermatids and the appearance of histochemical activity of 11 beta-HSDH in the Leydig cells seem to be closely correlated. An additional observation concerning the presence of germ cell debris was made in the seminiferous tubules during the postnatal development. Large amounts of debris were observed only till day 33.
Authors:
A Neumann; S G Haider; B Hilscher
Related Documents :
18550819 - Carboxypeptidase e mediates palmitate-induced beta-cell er stress and apoptosis.
9727839 - Hormonal factors from the mammalian pineal gland interfere with cell development in hydra.
6862099 - Lack of estradiol receptor in kurloff cell cytosol.
10864019 - Immunohistochemical and chemical changes of beta-citryl-l-glutamate in the differentiat...
7240739 - Inhibitory effect of k-76 monocarboxylic acid, an anticomplementary agent, on the c3b i...
17618499 - In vitro biological evaluation of beta-tcp/hdpe--a novel orthopedic composite: a survey...
10191409 - Allocation of atp to synthesis of cells and hydrolytic enzymes in cellulolytic fermenta...
21510739 - Differentiation of podocyte and proximal tubule-like cells from a mouse kidney-derived ...
10934789 - The influence of low h2o2 concentrations on dna content distribution in cell cycle and ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  25     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:    1993 Sep-Oct
Date Detail:
Created Date:  1993-12-29     Completed Date:  1993-12-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  263-9     Citation Subset:  IM    
Affiliation:
Department of Anatomy II, Heinrich-Heine-University Düsseldorf, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
11-beta-Hydroxysteroid Dehydrogenases
Age Factors
Animals
Histocytochemistry
Hydroxysteroid Dehydrogenases / metabolism*
Leydig Cells / enzymology*
Male
Rats
Seminiferous Epithelium / cytology
Sexual Maturation
Spermatids / cytology*
Testis / cytology,  enzymology
Chemical
Reg. No./Substance:
EC 1.1.-/Hydroxysteroid Dehydrogenases; EC 1.1.1.146/11-beta-Hydroxysteroid Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protection of spermatogenesis against cytotoxic effects of two chemotherapeutic drugs by temporary t...
Next Document:  Purification and characterization of sperm-coating antigen, identified by a monoclonal antibody.