| Temporal changes in mouse brain fatty acid amide hydrolase activity. | |
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MedLine Citation:
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PMID: 19555737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fatty acid amide hydrolase (FAAH) activity is known to mediate the tone of endogenous fatty acid amides including the endocannabinoid anandamide. FAAH is a potential therapeutic target because genetic or pharmacological ablation of FAAH promotes analgesia and anxiolytic effects without disrupting motor coordination. Little is known about the endogenous temporal fluctuations of brain FAAH activity. This is the first comprehensive study examining temporal fluctuations in mouse brain FAAH activity. Regional mouse brain homogenates were generated at the midpoint of the light ("noon") and dark ("midnight") cycles. While immunoblots revealed no significant changes (P>0.05) in regional activity between these two time points, in vitro activity assays detected a subtle 10% reduction (P<0.05) in cerebellar FAAH activity at midnight. A novel ex vivo autoradiography technique permitted the study of 11 different brain regions, many of which cannot be studied using traditional in vitro methods. The cerebellum and the periaqueductal gray both exhibited significant (P<0.05) reductions in regional FAAH activity in "midnight" brains. These data confirm the need to account for temporal changes in FAAH activity when therapeutically targeting FAAH. |
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Authors:
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S T Glaser; M Kaczocha |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-06-23 |
Journal Detail:
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Title: Neuroscience Volume: 163 ISSN: 1873-7544 ISO Abbreviation: Neuroscience Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-04 Completed Date: 2009-12-14 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: United States |
Other Details:
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Languages: eng Pagination: 594-600 Citation Subset: IM |
Affiliation:
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Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794-5230, USA. sherrye.glaser@stonybrook.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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metabolism* Animals Autoradiography Blotting, Western Brain / enzymology*, metabolism Cerebellum / enzymology, metabolism Circadian Rhythm* Mice Mice, Inbred C57BL Periaqueductal Gray / enzymology, metabolism Periodicity Photoperiod Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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1 K01 DA021806-01/DA/NIDA NIH HHS; K01 DA021806-03/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.5.-/Amidohydrolases; EC 3.5.1.-/fatty-acid amide hydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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