Document Detail


Temporal appearance of structural and nonstructural bluetongue viral proteins in infected cells, as determined by immunofluorescence staining and flow cytometry.
MedLine Citation:
PMID:  2167029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The temporal appearance of 4 viral proteins was detected in bluetongue virus (BTV)-infected Vero cells by indirect immunofluorescence staining with monoclonal antibodies (MAb) to BTV structural proteins VP2 and VP7 and nonstructural proteins NS1 and NS2. Bluetongue viral proteins were detected at distinct intervals after inoculation of Vero cells; VP7 was first detected 3 hours after inoculation, NS1 and NS2 at 5 hours after inoculation, whereas VP2 was not detected until 8 hours after inoculation. Patterns of fluorescence varied with the fixative used, but each MAb induced a distinct pattern of fluorescence in infected cells. Flow cytometry, which was used with each of the 4 MAb, proved to be an accurate and sensitive method of detecting BTV-infected P3 mouse myeloma cells. The temporal appearance of each viral protein in BTV-infected P3 cells was similar to that detected in BTV-infected Vero cells. Advantages of flow cytometry over conventional immunofluorescence staining to detect BTV-infected cells included: (1) enumeration of the proportion of infected cells in a population; (2) further characterization of infected cells, including estimates of their viability; and (3) computer-assisted storage and analysis of data obtained.
Authors:
L E Whetter; D H Gebhard; N J MacLachlan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of veterinary research     Volume:  51     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-20     Completed Date:  1990-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1174-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use*
Bluetongue virus*
Cell Separation
Flow Cytometry / methods
Fluorescent Antibody Technique
Reoviridae*
Time Factors
Vero Cells
Viral Proteins / analysis*,  biosynthesis
Viral Structural Proteins / analysis,  biosynthesis
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Viral Proteins; 0/Viral Structural Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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