| Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant mouse uterus. | |
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MedLine Citation:
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PMID: 20015327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: The tumor-associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is considered to play a role in the inhibition of maternal immune response during pregnancy, and participates in the initiation of labor and placental detachment. The aim of our study was to investigate the expression of RCAS1 protein in the uteri of normal pregnant mice. METHOD: of study Uteri with fetuses were collected from pregnant ICR mice on days 1.5, 3.5, 5.5, 7.5, and 9.5 p.c., and uterine and placental tissues were obtained separately on days 11.5, 13.5, 15.5, and 17.5 p.c. Samples were examined using real-time (RT)-PCR, Western blotting, and immunohistochemical analyses. RESULTS: In normal pregnant mice, RCAS1 protein mRNA was significantly increased on day 7.5 p.c. Antigen localization was detected in the placenta, decidua, and fetus. CONCLUSION: The results of this study suggest the importance of day 7.5 p.c. for RCAS1 protein expression in connection with placentation as a possible target for future in vivo studies. |
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Authors:
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Ekaterine Tskitishvili; Hitomi Nakamura; Yukiko Kinugasa-Taniguchi; Takeshi Kanagawa; Tadashi Kimura; Takuji Tomimatsu; Koichiro Shimoya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-15 |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 63 ISSN: 1600-0897 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-12 Completed Date: 2010-03-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 137-43 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan. ekaterinet@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Neoplasm / genetics*, metabolism* Blotting, Western Decidua / metabolism Female Fetus / metabolism Gene Expression Gestational Age Immunohistochemistry Mice Mice, Inbred ICR Placenta / metabolism Placentation / genetics, physiology Pregnancy Pregnancy, Animal / genetics*, metabolism* RNA, Messenger / genetics, metabolism Uterus / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/EBAG9 protein, mouse; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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