Document Detail


Temporal relationship between primary and motile ciliogenesis in airway epithelial cells.
MedLine Citation:
PMID:  20118219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cilia are traditionally classified as motile or primary. Motile cilia are restricted to specific populations of well-differentiated epithelial cells, including those in the airway, brain ventricles, and oviducts. Primary cilia are nonmotile, solitary structures that are present in many cell types, and often have sensory functions such as in the retina and renal tubules. Primary cilia were also implicated in the regulation of fundamental processes in development. Rare depictions of primary cilia in embryonic airways led us to hypothesize that primary cilia in airway cells are temporally related to motile ciliogenesis. We identified primary cilia in undifferentiated, cultured airway epithelial cells from mice and humans and in developing lungs. The solitary cilia in the airways express proteins considered unique to primary cilia, including polycystin-1 and polycystin-2. A temporal analysis of airway epithelial cell differentiation showed that cells with primary cilia acquire markers of motile ciliogenesis, suggesting that motile ciliated cells originate from primary ciliated cells. Whereas motile ciliogenesis requires Foxj1, primary ciliogenesis does not, and the expression of Foxj1 was associated with a loss of primary cilia, just before the appearance of motile cilia. Primary cilia were not found in well-differentiated airway epithelial cells. However, after injury, they appear in the luminal layer of epithelium and in basal cells. The transient nature of primary cilia, together with the temporal and spatial patterns of expression in the development and repair of airway epithelium, suggests a critical role of primary cilia in determining outcomes during airway epithelial cell differentiation.
Authors:
Raksha Jain; Jiehong Pan; James A Driscoll; Jeffrey W Wisner; Tao Huang; Sean P Gunsten; Yingjian You; Steven L Brody
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-01-29
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  43     ISSN:  1535-4989     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-01-05     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  731-9     Citation Subset:  IM    
Affiliation:
Division of Pulmonary and Critical Care, Department of Internal Medicine, Washington University School of Medicine, 660 South Euclid Ave., St. Louis, MO 63110, USA.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism,  pathology
Animals
Cell Line
Cell Proliferation
Cells, Cultured
Cilia / metabolism*,  ultrastructure
Dogs
Epithelial Cells / metabolism*,  pathology,  ultrastructure
Forkhead Transcription Factors / metabolism
Humans
Lung Injury / metabolism,  pathology
Mice
Models, Biological
Movement / physiology*
Organogenesis*
TRPP Cation Channels / metabolism
Time Factors
Trachea / cytology*,  embryology*
Grant Support
ID/Acronym/Agency:
K08 HL105671/HL/NHLBI NIH HHS; K30 RR022251/RR/NCRR NIH HHS; R01 HL56244/HL/NHLBI NIH HHS; T32 HL07317/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/FOXJ1 protein, mouse; 0/Forkhead Transcription Factors; 0/TRPP Cation Channels
Comments/Corrections

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