Document Detail


Temperature-sensitive mutations of the CKA1 gene reveal a role for casein kinase II in maintenance of cell polarity in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  9488724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Casein kinase II (CKII) of Saccharomyces cerevisiae contains two distinct catalytic subunits, alpha and alpha', that are encoded by the CKA1 and -2 genes, respectively. We have constructed conditional alleles of the CKA1 gene. In contrast to cka1 cka2(ts) strains, which exhibit a defect in both G1 and G2/M cell cycle progression, cka1(ts) cka2 strains continue to divide for three cell cycles after a shift to restrictive temperature and then arrest as a mixture of budded and unbudded cells with a spherical morphology. Arrested cells exhibit continued growth, a nonpolarized actin cytoskeleton, delocalized chitin deposition, and a significant fraction of multinucleate cell bodies, confirming the presence of a cell polarity defect in cka1(ts) strains. The presence of budded as well as unbudded cells in the arrested population suggests that CKII is required for maintenance rather than establishment of cell polarity, although a role in both processes is also possible. The terminal phenotype of cka1(ts) strains bears a strong resemblance to that of orb5 strains of Schizosaccharomyces pombe, which carry a temperature-sensitive CKII catalytic subunit mutation, but the underlying mechanism appears to be different in the two cases. These results establish a requirement for CKII in cell polarity in S. cerevisiae and provide the first evidence for functional specialization of CKA1 and -2.
Authors:
A Rethinaswamy; M J Birnbaum; C V Glover
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  273     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-07     Completed Date:  1998-04-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5869-77     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, the University of Georgia, Athens, Georgia 30602-7229, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Amino Acid Sequence
Casein Kinase II
Cell Cycle / physiology
Cell Polarity / genetics,  physiology*
Chitin / metabolism
Chromatin / metabolism
Genes, Fungal / genetics
Molecular Sequence Data
Mutagenesis, Site-Directed / genetics
Mutation / genetics*
Phenotype
Protein-Serine-Threonine Kinases / genetics,  physiology*
Saccharomyces cerevisiae / genetics*
Sequence Deletion / genetics
Temperature
Grant Support
ID/Acronym/Agency:
GM33237/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Chromatin; 1398-61-4/Chitin; EC 2.7.11.1/Casein Kinase II; EC 2.7.11.1/Protein-Serine-Threonine Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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