| Temperature-sensitive mutations of the CKA1 gene reveal a role for casein kinase II in maintenance of cell polarity in Saccharomyces cerevisiae. | |
MedLine Citation:
|
PMID: 9488724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Casein kinase II (CKII) of Saccharomyces cerevisiae contains two distinct catalytic subunits, alpha and alpha', that are encoded by the CKA1 and -2 genes, respectively. We have constructed conditional alleles of the CKA1 gene. In contrast to cka1 cka2(ts) strains, which exhibit a defect in both G1 and G2/M cell cycle progression, cka1(ts) cka2 strains continue to divide for three cell cycles after a shift to restrictive temperature and then arrest as a mixture of budded and unbudded cells with a spherical morphology. Arrested cells exhibit continued growth, a nonpolarized actin cytoskeleton, delocalized chitin deposition, and a significant fraction of multinucleate cell bodies, confirming the presence of a cell polarity defect in cka1(ts) strains. The presence of budded as well as unbudded cells in the arrested population suggests that CKII is required for maintenance rather than establishment of cell polarity, although a role in both processes is also possible. The terminal phenotype of cka1(ts) strains bears a strong resemblance to that of orb5 strains of Schizosaccharomyces pombe, which carry a temperature-sensitive CKII catalytic subunit mutation, but the underlying mechanism appears to be different in the two cases. These results establish a requirement for CKII in cell polarity in S. cerevisiae and provide the first evidence for functional specialization of CKA1 and -2. |
Authors:
|
A Rethinaswamy; M J Birnbaum; C V Glover |
Related Documents
:
|
8995664 - S-phase-dependent cell cycle disturbances caused by aleutian mink disease parvovirus. 9207224 - Association of protein phosphatase-1delta with the retinoblastoma protein and reversibl... 15203384 - Individual and interactive effects of apigenin analogs on g2/m cell-cycle arrest in hum... 2843984 - An origin unwinding activity regulates initiation of dna replication during mammalian c... 10820234 - Heterogeneity of tonsillar subepithelial b lymphocytes, the splenic marginal zone equiv... 7003674 - Mechanism of action of antifungal drugs, with special reference to the imidazole deriva... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 273 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1998 Mar |
Date Detail:
|
Created Date: 1998-04-07 Completed Date: 1998-04-07 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 5869-77 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, the University of Georgia, Athens, Georgia 30602-7229, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actins
/
metabolism Amino Acid Sequence Casein Kinase II Cell Cycle / physiology Cell Polarity / genetics, physiology* Chitin / metabolism Chromatin / metabolism Genes, Fungal / genetics Molecular Sequence Data Mutagenesis, Site-Directed / genetics Mutation / genetics* Phenotype Protein-Serine-Threonine Kinases / genetics, physiology* Saccharomyces cerevisiae / genetics* Sequence Deletion / genetics Temperature |
| Grant Support | |
ID/Acronym/Agency:
|
GM33237/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 0/Chromatin; 1398-61-4/Chitin; EC 2.7.11.1/Casein Kinase II; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139.
Next Document: Molecular characterization of the 50- and 57-kDa subunits of the bovine vacuolar proton pump.