Document Detail

Temperature sculpting in yoctoliter volumes.
MedLine Citation:
PMID:  23347384     Owner:  NLM     Status:  MEDLINE    
The ability to perturb large ensembles of molecules from equilibrium led to major advances in understanding reaction mechanisms in chemistry and biology. Here, we demonstrate the ability to control, measure, and make use of rapid temperature changes in fluid volumes that are commensurate with the size of single molecules. The method is based on attaching gold nanoparticles to a single nanometer-scale pore formed by a protein ion channel. Visible laser light incident on the nanoparticles causes a rapid and large increase of the adjacent solution temperature, which is estimated from the change in the nanopore ionic conductance. The temperature shift also affects the ability of individual molecules to enter into and interact with the nanopore. This technique could significantly improve sensor systems and force measurements based on single nanopores, thereby enabling a method for single molecule thermodynamics and kinetics.
Joseph E Reiner; Joseph W F Robertson; Daniel L Burden; Lisa K Burden; Arvind Balijepalli; John J Kasianowicz
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Publication Detail:
Type:  Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-14
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  135     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-08-12     Revised Date:  2014-07-28    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3087-94     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Gold / chemistry
Metal Nanoparticles / chemistry
Microscopy, Electron, Scanning
Proteins / chemistry*
Grant Support
Reg. No./Substance:
0/Proteins; 7440-57-5/Gold
Erratum In:
J Am Chem Soc. 2014 Apr 2;136(13):5177

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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