Document Detail

Temperature-responsive chromatography using poly(N-isopropylacrylamide) hydrogel-modified silica.
MedLine Citation:
PMID:  11817726     Owner:  NLM     Status:  MEDLINE    
Poly(N-isopropylacrylamide) (PNIPAAm) has the sharpest phase transition of the class of thermo-sensitive N-alkyl acrylamide polymers. We developed a new method of HPLC using packing materials modified with cross-linked poly(N-isopropylacrylamide) (PNIPAAm) hydrogel. A temperature-responsive surface was prepared by polymerization of NIPAAm in the presence of a cross-linker on the silica support. The surface properties and functions of the stationary phases change in response to the external temperature. Therefore it easily changes the interaction of a solute with the surface with a constant aqueous mobile phase. A temperature-responsive elution behavior was observed on the separation of steroids and PTH-amino acids. The method is expected to be applicable to separation in the pharmaceutical and biomedical fields.
Hideko Kanazawa; Tatsuo Sunamoto; Eri Ayano; Yoshikazu Matsushima; Akihiko Kikuchi; Teruo Okano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  18     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-30     Completed Date:  2002-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  45-8     Citation Subset:  IM    
Kyoritsu College of Pharmacy,Minato, Tokyo, Japan.
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MeSH Terms
Chromatography, High Pressure Liquid / methods
Indicators and Reagents
Reg. No./Substance:
0/Acrylamides; 0/Acrylates; 0/Indicators and Reagents; 0/N-isopropylacrylamide-acrylic acid copolymer; 0/Polymers

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