| Temperature-dependent phosphorylation state of the H5R protein synthesised at the early stage of infection in cells infected with vaccinia virus ts mutants of the B1R and F10L protein kinases. | |
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MedLine Citation:
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PMID: 10773740 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The phosphorylation state of H5R protein was investigated by two-dimensional gel electrophoresis of proteins of BSC-40 cells infected at 32 degrees or 39.5 degrees with vaccinia virus ts mutants of the viral B1R or F10L protein kinase genes. A temperature-dependent increase of underphosphorylated H5R protein (pI 6.8) was demonstrated in the case of the B1R, but not of the F10L gene. The temperature-dependent cytoplasmic location of underphosphorylated H5R protein after infection with the ts mutants of the B1R gene was the consequence of the associated viral DNA replication block. These results show that the B1R protein kinase controls the phosphorylation state of the H5R protein synthesised at the early stage in vaccinia-virus-infected cells. |
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Authors:
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G Beaud; R Beaud |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Intervirology Volume: 43 ISSN: 0300-5526 ISO Abbreviation: Intervirology Publication Date: 2000 |
Date Detail:
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Created Date: 2000-06-21 Completed Date: 2000-06-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364265 Medline TA: Intervirology Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 67-70 Citation Subset: IM |
Copyright Information:
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Copyright 2000 S. Karger AG, Basel. |
Affiliation:
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Institut Jacques-Monod, CNRS, Université Pierre-et-Marie-Curie (Paris 6) et Université Denis-Diderot (Paris 7), Paris, France. beaud@ijm.jussieu.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cercopithecus aethiops DNA Replication DNA-Binding Proteins / metabolism* Mutation Phosphorylation Protein-Serine-Threonine Kinases / genetics, metabolism* Temperature Vaccinia virus / genetics, metabolism* Viral Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/H5R protein, Vaccinia virus; 0/VB1R protein, Vaccinia virus; 0/Viral Proteins; EC 2.7.1.-/protein kinase 2, Vaccinia virus; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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