Document Detail


Temperature-dependence of LDL binding and activation of human platelets.
MedLine Citation:
PMID:  1788835     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of low density lipoprotein (LDL) on intracellular free calcium ion concentration ([Ca2+]i), taken as an index of the degree of platelet activation, was investigated in normal volunteers. At 37 degrees C LDL, in a dose of 20 micrograms of protein/ml, increased [Ca2+]i in all subjects tested (basal 57 +/- 11 to 113 +/- 19 nM). In contrast, when measurements were performed at 20 degrees C, no effect on [Ca2+]i was seen following LDL. Thrombin (0.2 U/ml) increased [Ca2+]i to 455 +/- 98 nM. When platelets had been exposed to LDL before thrombin stimulation, this increase was less pronounced (to 301 +/- 43 nM). Our finding of a temperature dependence of LDL induced increase in platelet [Ca2+]i supports the concept of a platelet-LDL receptor mediated mechanism. Furthermore, the lower thrombin response following LDL exposure suggests a LDL-thrombin interaction, possibly at the thrombin receptor level and/or calcium recruitment from the same stores.
Authors:
P L Katzman; R Bose; S Walker; Y Perry; P Bolli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis research     Volume:  64     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-03-20     Completed Date:  1992-03-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  503-8     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adult
Blood Platelets / metabolism*
Calcium / blood
Humans
Intracellular Fluid / chemistry
Lipoproteins, LDL / metabolism*
Middle Aged
Platelet Activation*
Temperature
Thrombin / metabolism
Chemical
Reg. No./Substance:
0/Lipoproteins, LDL; 7440-70-2/Calcium; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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