Document Detail

Temperature Dependence of the Production of Staphylococcal Enterotoxin A by Staphylococcus aureus.
MedLine Citation:
PMID:  23291745     Owner:  NLM     Status:  Publisher    
We incubated 11 strains of Staphylococcus aureus in a brain heart infusion broth at 10-37 °C with two inoculum sizes and examined their enterotoxin A (SEA) production by a Western blot analysis to clarify the effect of incubation temperature on SEA production. Although SEA was detected in the exponential phase at 15-37 °C, it was also detected in the stationary or death phase at 10 °C. The maximal SEA concentrations of most strains increased as the temperature was increased, although some strains produced as much at 15 °C and 20 °C as they did at 37 °C. The maximal SEA concentration was definitely lowest at 10 °C, and as the temperature was increased, the production rate increased. However, a relationship between the production rates at the two different temperatures was not apparent. Some strains produced more SEA at 10-20 °C with a smaller inoculum size than with a larger one. SEA production therefore did not necessarily depend on the incubation temperature, and it would be difficult to predict at 10 °C and 15 °C from the production at 37 °C.
Satomi Tsutsuura; Yuko Shimamura; Masatsune Murata
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  -     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Nutrition and Food Science, Ochanomizu University.
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