| Telomeric profiles and telomerase activity in turkey satellite cell clones with different in vitro growth characteristics. | |
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MedLine Citation:
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PMID: 11004508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The satellite cell population in postnatal skeletal muscle is heterogeneous because individual satellite cells isolated from a single muscle have differing abilities to proliferate under the same in vitro conditions. Telomeres are structures found at the ends of all eukaryotic chromosomes that are characterized by repetitive DNA sequences, and they are important in determining cellular proliferation potential. The relationship between satellite cell proliferative heterogeneity and telomeric DNA was examined by digesting genomic DNA from large-colony-forming and small-colony-forming turkey satellite cell clones with HinfI, separating the restriction fragments on an agarose gel, and hybridizing the gels with an oligonucleotide probe specific for telomeric DNA. Turkey satellite cells generated telomeric restriction fragments up to approximately 180 kB. The large-colony-forming satellite cell clones had a larger proportion (P<0.05) of total telomeric restriction fragments below 33 kB than the small-colony-forming satellite cell clones. However, telomerase expression was detected in cultures from large-colony-forming and small-colony-forming turkey satellite cells suggesting that the differences in telomeric restriction fragments may not be related to the differences in in vitro proliferative behavior and that telomerase may contribute to the high in vitro growth capacity of turkey satellite cells. |
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Authors:
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P E Mozdziak; D C McFarland; E Schultz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1492 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-10-19 Completed Date: 2000-10-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 362-8 Citation Subset: IM |
Affiliation:
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Department of Poultry Science, North Carolina State University, Raleigh 27695, USA. pemozdzi@unity.ncsu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Southern Cells, Cultured Clone Cells Colony-Forming Units Assay DNA Fragmentation DNA Restriction Enzymes / metabolism Electrophoresis, Gel, Pulsed-Field Muscle Development Muscle, Skeletal / cytology*, growth & development Telomerase / genetics*, metabolism Telomere / genetics* Turkeys |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.49/Telomerase; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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