Document Detail


Telomeres are shorter in placental trophoblasts of pregnancies complicated with intrauterine growth restriction (IUGR).
MedLine Citation:
PMID:  20619976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Telomeres are nucleoprotein structures located at the termini of chromosomes, and protect them from fusion and degradation. Telomeres are progressively shortened with each mitotic cycle and by environmental factors. We hypothesized that antepartum stress can lead to accelerated telomere shortening in placental trophoblasts, and plays a role in intrauterine growth restriction (IUGR).
METHODS: Placental biopsies were derived from 16 pregnancies complicated with IUGR and from 13 uncomplicated pregnancies. Fluorescence-in-situ protocol was used to determine telomere length. Immunohistochemistry for hTERT was performed to assess telomerase activity. Clinical and histopathological characteristics were collected to ensure that IUGR was secondary to placental insufficiency. Fluorescence-in-situ-hybridization was used to rule out aneuploidy as a reason for shortened telomeres.
RESULTS: The number and intensity of telomeres staining and telomerase activity were significantly lower in the IUGR placentas. No aneuploidy was detected for the chromosomes checked in the placental biopsies.
CONCLUSIONS: Telomeres are shorter in trophoblasts of IUGR placentas.
Authors:
Tal Biron-Shental; Rivka Sukenik Halevy; Lilach Goldberg-Bittman; Dvora Kidron; Moshe D Fejgin; Aliza Amiel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Early human development     Volume:  86     ISSN:  1872-6232     ISO Abbreviation:  Early Hum. Dev.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708381     Medline TA:  Early Hum Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  451-6     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Ltd. All rights reserved.
Affiliation:
Department of OBGYN, Meir Medical Center, Kfar Saba, Israel.
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MeSH Terms
Descriptor/Qualifier:
Female
Fetal Growth Retardation / etiology,  genetics*
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Placental Insufficiency / genetics*
Pregnancy
Telomere / ultrastructure*
Trophoblasts / ultrastructure*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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