| Telomere shortening is proportional to the size of the G-rich telomeric 3'-overhang. | |
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MedLine Citation:
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PMID: 10787419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most normal diploid human cells do not express telomerase activity and are unable to maintain telomere length with ongoing cell divisions. We show that the length of the single-stranded G-rich telomeric 3'-overhang is proportional to the rate of shortening in four human cell types that exhibit different rates of telomere shortening in culture. These results provide direct evidence that the size of the G-rich overhang is not fixed but subject to regulation. The potential ability to manipulate this rate has profound implications both for slowing the rate of replicative aging in normal cells and for accelerating the rate of telomere loss in cancer cells in combination with anti-telomerase therapies. |
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Authors:
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K E Huffman; S D Levene; V M Tesmer; J W Shay; W E Wright |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 275 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-08-10 Completed Date: 2000-08-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 19719-22 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas 75083, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Breast
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metabolism Cell Aging* Cells, Cultured DNA / metabolism Endothelium, Vascular / ultrastructure Epithelium / ultrastructure Fibroblasts / ultrastructure Guanine / chemistry Humans Lung / metabolism Models, Genetic Telomere / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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AG01228/AG/NIA NIH HHS; GM47898/GM/NIGMS NIH HHS; GM55871/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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73-40-5/Guanine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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