| Telomere shortening impairs organ regeneration by inhibiting cell cycle re-entry of a subpopulation of cells. | |
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MedLine Citation:
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PMID: 12881434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomere shortening limits the regenerative capacity of primary cells in vitro by inducing cellular senescence characterized by a permanent growth arrest of cells with critically short telomeres. To test whether this in vitro model of cellular senescence applies to impaired organ regeneration induced by telomere shortening in vivo, we monitored liver regeneration after partial hepatectomy in telomerase-deficient mice. Our study shows that telomere shortening is heterogeneous at the cellular level and inhibits a subpopulation of cells with critically short telomeres from entering the cell cycle. This subpopulation of cells with impaired proliferative capacity shows senescence-associated beta-galactosidase activity, while organ regeneration is accomplished by cells with sufficient telomere reserves that are capable of additional rounds of cell division. This study provides experimental evidence for the existence of an in vivo process of cellular senescence induced by critical telomere shortening that has functional impact on organ regeneration. |
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Authors:
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A Satyanarayana; S U Wiemann; J Buer; J Lauber; K E J Dittmar; T Wüstefeld; M A Blasco; M P Manns; K L Rudolph |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 22 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-07-25 Completed Date: 2003-09-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 4003-13 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Division Immunohistochemistry Liver / cytology, ultrastructure Mice Mice, Knockout Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction RNA / genetics, physiology Regeneration* Telomerase / genetics, physiology Telomere* |
| Chemical | |
Reg. No./Substance:
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0/telomerase RNA; 63231-63-0/RNA; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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