Document Detail

Telomere metabolism and diagnostic demonstration of telomere measurement in the human esophagus for distinguishing benign from malignant tissue by tissue quantitative fluorescence in situ hybridization.
MedLine Citation:
PMID:  17878747     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: We have developed a novel method for evaluating telomere length in four different cell types in non-cancerous and cancerous mucosal tissue from 15 cases of squamous cell carcinoma of the esophagus using tissue quantitative fluorescence in situ hybridization (Q-FISH). We hypothesized that the very rapid cell proliferation observed in esophageal squamous cell carcinomas might accelerate the telomere shortening and chromosomal instability associated with carcinogenesis. METHODS: Tissue Q-FISH and the telomere to centromere intensity ratio (TCR) were used to compare telomere shortening in tissue sections taken from esophageal squamous cell carcinomas and adjacent non-cancerous esophageal tissues. RESULTS: The peak percentage of TCR was <1 for esophageal squamous carcinoma cells and >1 for the non-cancerous esophageal cell types. Basal layer cells had the longest telomeres in comparison with prickle, cancer, and stromal cells, and strongly expressed hTERT, cytokeratin 14 and CD49f, but not MIB-1. CONCLUSION: These results suggest the presence of stem cells in the basal layer of the esophagus. Esophageal squamous cell carcinomas also display anaphase bridges, evidencing chromosomal instability. In conclusion, our TCR method can be used to distinguish between benign and malignant tissue in esophageal lesions. In order to apply this approach clinically to individual cases, further studies are in progress.
Makoto Kammori; Naotaka Izumiyama; Ken-ichi Nakamura; Rie Kurabayashi; Mitsuhiko Kashio; Junko Aida; Steven S S Poon; Michio Kaminishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-18
Journal Detail:
Title:  Oncology     Volume:  71     ISSN:  1423-0232     ISO Abbreviation:  Oncology     Publication Date:  2006  
Date Detail:
Created Date:  2007-10-15     Completed Date:  2007-11-09     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0135054     Medline TA:  Oncology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  430-6     Citation Subset:  IM    
Copyright Information:
Copyright 2006 S. Karger AG, Basel.
Department of Breast, Tokyo Metropolitan Tama Cancer Detection Center, Fuchu-shi, Tokyo, Japan.
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MeSH Terms
Anaphase / genetics
Carcinoma, Squamous Cell / diagnosis*,  genetics,  metabolism
Centromere / pathology
Chromosomal Instability / genetics
Esophageal Neoplasms / diagnosis*,  genetics,  metabolism
Esophagus / metabolism,  pathology*
In Situ Hybridization, Fluorescence / methods*
Middle Aged
Stem Cells / pathology
Telomere / genetics*,  metabolism,  pathology
Tumor Markers, Biological / biosynthesis
Reg. No./Substance:
0/Tumor Markers, Biological

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