| Telomere maintenance in telomerase-deficient mouse embryonic stem cells: characterization of an amplified telomeric DNA. | |
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MedLine Citation:
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PMID: 10805753 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomere dynamics, chromosomal instability, and cellular viability were studied in serial passages of mouse embryonic stem (ES) cells in which the telomerase RNA (mTER) gene was deleted. These cells lack detectable telomerase activity, and their growth rate was reduced after more than 300 divisions and almost zero after 450 cell divisions. After this growth crisis, survivor cells with a rapid growth rate did emerge. Such survivors were found to maintain functional telomeres in a telomerase-independent fashion. Although telomerase-independent telomere maintenance has been reported for some immortalized mammalian cells, its molecular mechanism has not been elucidated. Characterization of the telomeric structures in one of the survivor mTER(-/-) cell lines showed amplification of the same tandem arrays of telomeric and nontelomeric sequences at most of the chromosome ends. This evidence implicates cis/trans amplification as one mechanism for the telomerase-independent maintenance of telomeres in mammalian cells. |
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Authors:
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H Niida; Y Shinkai; M P Hande; T Matsumoto; S Takehara; M Tachibana; M Oshimura; P M Lansdorp; Y Furuichi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 20 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-07-06 Completed Date: 2000-07-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4115-27 Citation Subset: IM |
Affiliation:
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Agene Research Institute, Kamakura 247-0063, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB040048; AB040049 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Survival Cloning, Molecular DNA DNA, Complementary Mice Molecular Sequence Data Stem Cells Telomerase / genetics, physiology* Telomere / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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GM56162/GM/NIGMS NIH HHS; R0IAI29524/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 9007-49-2/DNA; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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