| Telomere length variations in 6 mucosal cell types of gastric tissue observed using a novel quantitative fluorescence in situ hybridization method. | |
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MedLine Citation:
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PMID: 17588641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a novel method for evaluating telomere length in 6 cell types of noncancerous and cancerous mucosal tissues from 11 cases of gastric neoplasm using the quantitative fluorescence in situ hybridization method with telomere and centromere peptide nucleic acid probes. Our telomere length estimates were determined from the background-corrected telomere intensity divided by the background-corrected centromere intensity (telomere-to-centromere ratio). Our results indicated that telomere lengths in each of the cases studied were reduced in turn from fibroblasts to fundic gland cells, to glandular neck cells, and then to surface foveolar cells. However, the telomere lengths of intestinalized cells located among fundic glands were not always shorter than those of the other cell types, as reported previously by others. Helicobacter pylori infection was suggested to induce the telomere shortening seen in the fundic glands. Although the mean telomere lengths varied among the 8 gastric cancer cases, correlation of the telomere lengths with the Ki-67 labeling index was established after normalization with the fibroblast measurements. We conclude that our telomere-to-centromere ratio method can reliably estimate the telomere lengths of the 6 cell types in the gastric mucosa and clarifies the relationship between proliferative activity and the telomere length of cancer cells. |
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Authors:
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Junko Aida; Naotaka Izumiyama-Shimomura; Ken-Ichi Nakamura; Akio Ishii; Naoshi Ishikawa; Naoko Honma; Rie Kurabayashi; Makoto Kammori; Steven S S Poon; Tomio Arai; Kaiyo Takubo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-22 |
Journal Detail:
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Title: Human pathology Volume: 38 ISSN: 0046-8177 ISO Abbreviation: Hum. Pathol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-20 Completed Date: 2007-09-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421547 Medline TA: Hum Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 1192-200 Citation Subset: IM |
Affiliation:
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Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan. aidajs@tmig.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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genetics,
metabolism*,
pathology Aged Aged, 80 and over Cell Proliferation Centromere / genetics, metabolism Female Gastric Mucosa / metabolism*, pathology Helicobacter Infections / genetics, metabolism, pathology Humans Immunoenzyme Techniques In Situ Hybridization, Fluorescence* Ki-67 Antigen / metabolism Male Oligonucleotide Probes / analysis, genetics Peptide Nucleic Acids / analysis, genetics Stomach Neoplasms / genetics, metabolism*, pathology Telomere / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ki-67 Antigen; 0/Oligonucleotide Probes; 0/Peptide Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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