Document Detail


Telomere length is reset during early mammalian embryogenesis.
MedLine Citation:
PMID:  15148368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The enzyme telomerase is active in germ cells and early embryonic development and is crucial for the maintenance of telomere length. Whereas the different length of telomeres in germ cells and somatic cells is well documented, information on telomere length regulation during embryogenesis is lacking. In this study, we demonstrate a telomere elongation program at the transition from morula to blastocyst in mice and cattle that establishes a specific telomere length set point during embryogenesis. We show that this process restores telomeres in cloned embryos derived from fibroblasts, regardless of the telomere length of donor nuclei, and that telomere elongation at this stage of embryogenesis is telomerase-dependent because it is abrogated in telomerase-deficient mice. These data demonstrate that early mammalian embryos have a telomerase-dependent genetic program that elongates telomeres to a defined length, possibly required to ensure sufficient telomere reserves for species integrity.
Authors:
Sonja Schaetzlein; Andrea Lucas-Hahn; Erika Lemme; Wilfried A Kues; Martina Dorsch; Michael P Manns; Heiner Niemann; K Lenhard Rudolph
Related Documents :
3740938 - Lymphocyte proliferation and nucleoid sedimentation in a case of premature aging distin...
1119348 - The use of arrested populations of human diploid fibroblasts for the study of senescenc...
16671998 - Spontaneous senescence in the mda-mb-231 cell line.
12373308 - Immortal phenotype of the esophageal epithelial cells in the process of immortalization.
14597578 - Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in sm...
17431708 - The environmental plasmid pqbr103 alters the single-cell raman spectral profile of pseu...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-05-17
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  101     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-26     Completed Date:  2004-07-09     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8034-8     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, Hepatology, and Endocrinology, and Institute for Animal Science, Hannover Medical School, 30625 Hannover, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / metabolism
Cattle
Embryonic and Fetal Development / genetics*
Gene Deletion
In Situ Hybridization, Fluorescence
Mammals / genetics
Mice
Mice, Knockout
Morula / metabolism
RNA / genetics
Telomerase / deficiency,  genetics
Telomere / chemistry*,  genetics*
Time Factors
Chemical
Reg. No./Substance:
0/telomerase RNA; 63231-63-0/RNA; EC 2.7.7.49/Telomerase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Activity-based probes for protein tyrosine phosphatases.
Next Document:  Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphas...