| Telomere length measurement by fluorescence in situ hybridization and flow cytometry. | |
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MedLine Citation:
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PMID: 14976379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomere length is an important measure of cellular differentiation and progression to senescence. Flow cytometric assays for measuring telomere length have become an important adjunct to more laborious Southern blotting methods; telomere length can be estimated with considerable accuracy in small numbers of individual cells by flow cytometry, and can be measured in cell population subsets with simultaneous fluorescent immunophenotyping. In this chapter, we describe the standard flow cytometric assay for measuring telomere length, including the incorporation of fluorochrome-conjugated antibody immunolabeling for measurement in cell subsets. |
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Authors:
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Veena Kapoor; William G Telford |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 263 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-02-20 Completed Date: 2004-07-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 385-98 Citation Subset: IM |
Affiliation:
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Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD4
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biosynthesis CD4-Positive T-Lymphocytes / metabolism Calibration Cell Membrane / metabolism Cross-Linking Reagents / pharmacology Flow Cytometry / methods* Humans Immunologic Memory In Situ Hybridization, Fluorescence / methods* Jurkat Cells Peptide Nucleic Acids / chemistry Peptides / chemistry Phenotype T-Lymphocytes / metabolism Telomerase / metabolism Telomere / ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD4; 0/Cross-Linking Reagents; 0/Peptide Nucleic Acids; 0/Peptides; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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