Document Detail


Telomere length inversely correlates with radiosensitivity in human carcinoma cells with the same tissue background.
MedLine Citation:
PMID:  18155157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A relationship between telomeres and radiosensitivity has been established by several studies based on non-mammalian model systems, mouse models, and few human genetic diseases. However, the relationship has not been proven in human carcinoma cells, which have more clinical significance than these other models. The present study aims to determine whether telomere length is related to radiosensitivity in human carcinoma cells, and to examine the influence of tissue or genetic background. Two HEp-2 larynx squamous carcinoma cell lines, eight hepatocellular carcinoma cell lines, and five breast cancer cell lines were used. Telomere length was determined by terminal restriction fragment (TRF) Southern blot analysis and cell survival was measured by a colony-forming assay. Our results indicated that there was a significant negative correlation of telomere length and radiosensitivity in the same tissue-derived cell lines, with or without the same genetic background. Thus, telomere length may be used as a promising tool to predict the radiosensitivity of human carcinomas.
Authors:
Ya-Hua Zhong; Zheng-Kai Liao; Fu-Xiang Zhou; Cong-Hua Xie; Chuang-Ying Xiao; Dong-Feng Pan; Zhi-Guo Luo; Shi-Quan Liu; Yun-Feng Zhou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-26
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  367     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-18     Completed Date:  2008-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  84-9     Citation Subset:  IM    
Affiliation:
Department of Chemo-Radiotherapy Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
Breast Neoplasms* / pathology,  therapy
Cell Line, Tumor / cytology,  pathology,  radiation effects
Cell Survival / physiology,  radiation effects
Colony-Forming Units Assay
Dose-Response Relationship, Radiation
Gamma Rays*
Humans
Laryngeal Neoplasms* / pathology,  therapy
Liver Neoplasms* / pathology,  therapy
Telomere / physiology,  radiation effects*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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