| Telomere length in paroxysmal nocturnal hemoglobinuria correlates with clone size. | |
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MedLine Citation:
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PMID: 17697745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To study if telomere length can be used as a surrogate marker for the mitotic history in normal and affected hematopoietic cells from patients with paroxysmal nocturnal hemoglobinuria (PNH). METHODS: The telomere length was measured by automated multicolor flow fluorescence in situ hybridization in glycosyl-phosphatidyl-inositol anchored protein (GPI)-negative and GPI-positive peripheral blood leukocytes. Eleven patients were studied, two with predominantly hemolytic PNH and nine with PNH associated with marrow failure. RESULTS: Telomere length in GPI-negative cells was significantly shorter than in GPI-positive cells of the same patient (p < 0.01, n = 11). The difference in telomere length (telomere length in GPI-positive minus telomere length in GPI-negative cells) correlated with the percentage of GPI-negative white blood cells. CONCLUSION: Our results support the hypothesis that telomere length is correlated to the replicative history of GPI-positive and GPI-negative cells and warrant further studies of telomere length in relation to disease progression in PNH. |
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Authors:
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Gabriela M Baerlocher; Elaine M Sloand; Neal S Young; Peter M Lansdorp |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-08-13 |
Journal Detail:
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Title: Experimental hematology Volume: 35 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-26 Completed Date: 2008-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1777-81 Citation Subset: IM |
Affiliation:
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Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Flow Cytometry Hemoglobinuria, Paroxysmal / genetics* Humans Telomere* |
| Grant Support | |
ID/Acronym/Agency:
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AI29524/AI/NIAID NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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