| Telomere attrition and chromosome instability via downregulation of TRF2 contributes to arsenic trioxide-induced apoptosis of human T-Cell leukemia cell line molt-4 cells. | |
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MedLine Citation:
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PMID: 17643074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Overexpression of human telomere repeat binding factor 2 (TRF2), which may play an important role in the fate of cancer cells, has been observed in adult T-cell leukemia. Previous reports have shown that the inhibition of TRF2 results in the apoptosis of cancer cells. In this study, we demonstrated that arsenic trioxide (As2O3) induced in vitro growth inhibition and/or apoptosis of human T-cell leukemia cell line Molt-4 in a caspase-independent manner. Telomerase activity was not inhibited, although the level of the reverse transcriptase subunit of the human telomerase gene (hTERT) mRNA expression was down regulated during the early times and then recovered to the level found in untreated controls about 48 hours after treatment with As2O3. Furthermore, a remarkable telomere shortening related to exposure of As2O3 was observed in 50 population doubling. Inc ontrast, the alteration of telomere length did not occur after exposure to higher concentration of As2O3 (10 microM) for 24 hours and 48 hours, respectively, suggesting that the shortening of telomeres induced by As2O3 is dependent of a series of cell division cycles. Chromosomal analysis showed that As2O3 exposure caused chromosomal end-to-end fusion in human T-cell leukemia cells while downregulation of TRF2 was observed. Finally, the inhibition of TRF2 protein expression and the sensitivity to As2O3 in a panel of leukemia cell lines were checked. The data revealed that inhibition of TRF2 rendered leukemia cells more susceptible to As2O3. In conclusion, the downregulation of TRF2 by As2O3 contribute to chromosomal end-to-end fusion, and apoptosis in leukemia cells, suggesting that TRF2 could be an attractive target for new therapies of leukemia. |
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Authors:
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Yangwen Jiao; Weifang Zhang; Junqing Liu; Wanmao Ni; Weilai Xu; Jie Jin; Wenbin Qian |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-04 |
Journal Detail:
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Title: Cancer biology & therapy Volume: 6 ISSN: 1555-8576 ISO Abbreviation: Cancer Biol. Ther. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-02-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101137842 Medline TA: Cancer Biol Ther Country: United States |
Other Details:
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Languages: eng Pagination: 1186-92 Citation Subset: IM |
Affiliation:
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Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis Arsenicals / pharmacology* Caspases / metabolism Cell Line, Tumor Cell Proliferation / drug effects Chromosomal Instability* Humans Leukemia-Lymphoma, Adult T-Cell / genetics, metabolism* Oxides / pharmacology* Telomerase / antagonists & inhibitors, genetics, metabolism Telomere / drug effects* Telomeric Repeat Binding Protein 2 / antagonists & inhibitors*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Arsenicals; 0/Oxides; 0/Telomeric Repeat Binding Protein 2; 1327-53-3/arsenic trioxide; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase; EC 3.4.22.-/Caspases |
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