Document Detail

Telomerase subunits expression variation between biopsy samples and cell lines derived from malignant glioma.
MedLine Citation:
PMID:  17196947     Owner:  NLM     Status:  MEDLINE    
Although scientific advances have recognised the prognostic power of telomerase activity in different cancers, as yet there has been no investigation regarding the expression variation of telomerase subunits in glioma tissues and cell lines. In this study, a recurrent anaplastic ependymoma and seven glioblastoma biopsy samples, four cell lines and four controls including two normal brain tissues were analysed for telomerase subunit expression profiles together with telomerase activity. Since telomerase activity is linked to tumourgenesis, the genes were analysed with respect to their expression variation. TEP1 was expressed in all glioma cell lines and 70% of glioblastoma tissues, in addition to the control brain tissues. Tankyrase was expressed in 85% of the glioblastoma tissues and was down-regulated in the recurrent anaplastic ependymoma tissue control cell lines. However, it was expressed in the control tissues. Dyskerin was expressed in all cell lines and tissues apart from U87-MG and NHA cells and the recurrent anaplastic ependymoma tissue. As expected, PARP1 and GAPDH showed constitutive expression throughout all cell lines and tissues since both are known to be housekeeping genes. hTERT was expressed in all glioma cell lines and tissues but was absent in the control cells and tissues. Telomerase activity was absent in IPDDC-A2 cells and 57% of the glioblastoma tissues. These results suggest that hTERT expression and not telomerase activity possibly represents a simple and reliable biological diagnostic tool.
Amal Shervington; Rahima Patel; Chen Lu; Nichola Cruickshanks; Robert Lea; Gareth Roberts; Tim Dawson; Leroy Shervington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-02
Journal Detail:
Title:  Brain research     Volume:  1134     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-29     Completed Date:  2007-04-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  45-52     Citation Subset:  IM    
Department of Biological Sciences, University of Central Lancashire, and Neurosurgery Department, Royal Preston Hospital, UK.
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MeSH Terms
Brain Neoplasms / diagnosis,  enzymology*,  genetics*
Carrier Proteins / genetics
Cell Cycle Proteins / genetics
Cell Line, Tumor
Cell Transformation, Neoplastic / genetics,  metabolism
Gene Expression Regulation, Neoplastic / genetics
Glioma / diagnosis,  enzymology*,  genetics*
Nuclear Proteins / genetics
Tankyrases / genetics
Telomerase / genetics*
Tumor Markers, Biological / genetics*
Reg. No./Substance:
0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DKC1 protein, human; 0/Nuclear Proteins; 0/TEP1 protein, human; 0/Tumor Markers, Biological; EC; EC protein, human; EC protein, human; EC

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