| Telomerase downregulation in cancer brain stem cell. | |
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MedLine Citation:
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PMID: 19430894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cancer stem cells (CSCs) are a minute sub-population of self-renewing, immortal cells, which can be responsible for chemoresistance observed in the treatment of cancer. CSCs are similar to cancer cells requiring telomerase activity or alternative mechanisms for their proliferation and regeneration. This study explored the correlation between CD133 (stem cell marker) and telomerase expression using CD133+ cells isolated from the glioma GOS-3 cell line with magnetic affinity cell sorting (MACS). GOS-3 CD133+ showed a transcription downregulation of hTERT ( approximately 100-fold decrease) compared with CD133- cells. In order to further substantiate the novel finding, serum deprivation was adopted to enrich CD133 expression in GOS-3 cells. A pronounced upregulation of cd133 and downregulation of telomerase expression were produced as a consequence of decreasing serum supplement levels in GOS-3 cells. These findings showed for the first time that telomerase is downregulated in brain cancer stem cells compared to cancer cells. |
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Authors:
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Amal Shervington; Chen Lu; Rahima Patel; Leroy Shervington |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-09 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 331 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-30 Completed Date: 2010-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 153-9 Citation Subset: IM |
Affiliation:
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Brain Tumour North West, Faculty of Science and Technology, University of Central Lancashire, Preston, UK. aashervington@uclan.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD Cell Line, Tumor Culture Media, Serum-Free Down-Regulation / genetics* Gene Expression Regulation, Neoplastic Glioma / enzymology*, genetics*, pathology Glycoproteins Humans Neoplastic Stem Cells / enzymology*, pathology Peptides RNA, Messenger / genetics, metabolism Telomerase / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/AC133 antigen; 0/Antigens, CD; 0/Culture Media, Serum-Free; 0/Glycoproteins; 0/Peptides; 0/RNA, Messenger; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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