Document Detail


Telomerase activity with concurrent loss of cell cycle regulation in feline post-traumatic ocular sarcomas.
MedLine Citation:
PMID:  16154137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Paraffin wax-embedded ocular globes of cats with post-traumatic ocular sarcomas were examined for the presence of TERT, the active subunit of telomerase. The latter is a ribonucleoprotein complex essential for immortalization and expressed by most malignant tumours, germ line cells, lens epithelial cells, and some stem cells. Due to the frequent loss of cell cycle control with the increased expression of telomerase activity, post-traumatic ocular sarcomas were also examined for loss of p16 expression and alterations in p53, the findings being related to mitotic score, tumour grade, and proliferating cell nuclear antigen. These sarcomas expressed telomerase at a high frequency (62.5%); in addition, the majority showed alterations in cell cycle control, as evaluated by lack of p16 immunolabelling (66.7%). Alterations in p53 were the sole mechanism by which cell cycle control was dysregulated in only two tumours expressing TERT (13%). These findings suggest that p16, and not p53, represents the primary mechanism by which post-traumatic ocular sarcomas that express telomerase activity escape cell cycle control.
Authors:
R T Carter; C Giudice; R R Dubielzig; C M H Colitz
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Publication Detail:
Type:  Journal Article     Date:  2005-09-08
Journal Detail:
Title:  Journal of comparative pathology     Volume:  133     ISSN:  0021-9975     ISO Abbreviation:  J. Comp. Pathol.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-07     Completed Date:  2005-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0102444     Medline TA:  J Comp Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  235-45     Citation Subset:  IM    
Affiliation:
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cat Diseases / enzymology*,  genetics,  pathology
Cats
Cell Cycle / genetics,  physiology*
DNA-Binding Proteins / genetics,  metabolism*
Eye / chemistry,  enzymology,  pathology
Eye Neoplasms / enzymology,  pathology,  veterinary*
Female
Gene Expression Regulation, Neoplastic
Genes, p16
Genes, p53
Male
Mitosis
Neoplasms, Post-Traumatic / enzymology,  pathology,  veterinary*
Proliferating Cell Nuclear Antigen / genetics,  metabolism
Sarcoma / enzymology,  pathology,  veterinary*
Telomerase / genetics,  metabolism*
Tumor Suppressor Protein p14ARF / genetics,  metabolism
Tumor Suppressor Protein p53 / genetics,  metabolism
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Proliferating Cell Nuclear Antigen; 0/Tumor Suppressor Protein p14ARF; 0/Tumor Suppressor Protein p53; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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