| Telomerase activity in human gliomas. | |
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MedLine Citation:
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PMID: 9588558 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Telomerase activity, which is undetectable in most mature normal tissues, has been identified in many types of human cancers, including neuroblastomas and oligodendrogliomas. These findings suggest that a novel mechanism in addition to activation of oncogenes and inactivation of tumor suppressor genes may play an important role in tumorigenesis. The goal of the present study was to assess and correlate the telomerase activity in astrocytic gliomas of different grades. METHODS: Telomere repeat amplification protocol and Southern blot hybridization with telomere-specific probes were used to detect telomerase activity and to measure terminal restriction fragment length, respectively. RESULTS: Telomerase activity was detected in 3 of 9 (33%) low-grade astrocytomas (World Health Organization Grade II), 5 of 11 (45%) anaplastic astrocytomas (World Health Organization Grade III), 36 of 41 (89%) glioblastomas multiforme (World Health Organization Grade IV), 3 of 4 (75%) oligodendrogliomas, and none of 4 normal brain specimens. CONCLUSION: We demonstrated that telomerase activity is absent in normal brain tissues while present in most glioma samples (72%). The frequency of such activity increases with malignancy. These results suggest that telomerase activity may be used as a tumor marker and that the activation of telomerase may correlate with initiation and malignant progression of astrocytic tumors. |
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Authors:
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S Le; J J Zhu; D C Anthony; C W Greider; P M Black |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neurosurgery Volume: 42 ISSN: 0148-396X ISO Abbreviation: Neurosurgery Publication Date: 1998 May |
Date Detail:
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Created Date: 1998-07-14 Completed Date: 1998-07-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7802914 Medline TA: Neurosurgery Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1120-4; discussion 1124-5 Citation Subset: IM |
Affiliation:
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Cold Spring Harbor Laboratory, New York, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Astrocytoma
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enzymology,
pathology Brain / enzymology* Brain Neoplasms / enzymology*, pathology DNA, Neoplasm / analysis Glioblastoma / enzymology, pathology Glioma / enzymology*, pathology Humans Neoplasm Proteins / analysis* Oligodendroglioma / enzymology, pathology Pilot Projects Telomerase / analysis* Telomere / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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AG09383/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Neoplasm Proteins; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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