Document Detail


Telomerase activity in human gliomas.
MedLine Citation:
PMID:  9588558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Telomerase activity, which is undetectable in most mature normal tissues, has been identified in many types of human cancers, including neuroblastomas and oligodendrogliomas. These findings suggest that a novel mechanism in addition to activation of oncogenes and inactivation of tumor suppressor genes may play an important role in tumorigenesis. The goal of the present study was to assess and correlate the telomerase activity in astrocytic gliomas of different grades. METHODS: Telomere repeat amplification protocol and Southern blot hybridization with telomere-specific probes were used to detect telomerase activity and to measure terminal restriction fragment length, respectively. RESULTS: Telomerase activity was detected in 3 of 9 (33%) low-grade astrocytomas (World Health Organization Grade II), 5 of 11 (45%) anaplastic astrocytomas (World Health Organization Grade III), 36 of 41 (89%) glioblastomas multiforme (World Health Organization Grade IV), 3 of 4 (75%) oligodendrogliomas, and none of 4 normal brain specimens. CONCLUSION: We demonstrated that telomerase activity is absent in normal brain tissues while present in most glioma samples (72%). The frequency of such activity increases with malignancy. These results suggest that telomerase activity may be used as a tumor marker and that the activation of telomerase may correlate with initiation and malignant progression of astrocytic tumors.
Authors:
S Le; J J Zhu; D C Anthony; C W Greider; P M Black
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurosurgery     Volume:  42     ISSN:  0148-396X     ISO Abbreviation:  Neurosurgery     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-14     Completed Date:  1998-07-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7802914     Medline TA:  Neurosurgery     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1120-4; discussion 1124-5     Citation Subset:  IM    
Affiliation:
Cold Spring Harbor Laboratory, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Astrocytoma / enzymology,  pathology
Brain / enzymology*
Brain Neoplasms / enzymology*,  pathology
DNA, Neoplasm / analysis
Glioblastoma / enzymology,  pathology
Glioma / enzymology*,  pathology
Humans
Neoplasm Proteins / analysis*
Oligodendroglioma / enzymology,  pathology
Pilot Projects
Telomerase / analysis*
Telomere / chemistry
Grant Support
ID/Acronym/Agency:
AG09383/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Neoplasm Proteins; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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