Document Detail


Telomerase activity in HeLa cervical carcinoma cell line proliferation.
MedLine Citation:
PMID:  16955216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal human somatic cells in culture have a limited dividing potential. This is due to DNA end replication problem, whereby telomeres shorten with each subsequent cell division. When a critical telomere length is reached cells enter senescence. To overcome this problem, immortal HeLa cell line express telomerase, an enzyme that prevents telomere shortening. Although immortal, the existence of non-dividing cells that do not incorporate (3)H-thymidine over 24 h of growth has been well documented in this cell line. Using DiI labeling and high-speed cell sorting, we have separated and analyzed fractions of HeLa cells that divided vigorously as well as those that cease divisions over several days in culture. We also analyzed telomerase activity in separated fractions and surprisingly, found that the fraction of cells that divided 0-1 time over 6 days in culture have several times higher endogenous telomerase activity than the fastest dividing fraction. Additionally, the non-growing fraction regains an overall high labeling index and low SA-beta-Gal activity when subcultured again. This phenomenon should be considered if telomerase inhibition is to be used as an approach to cancer therapy. In this paper we also discuss possible molecular mechanisms that underlie the observed results.
Authors:
Milena Ivanković; Andrea Cukusić; Ivana Gotić; Nikolina Skrobot; Mario Matijasić; Denis Polancec; Ivica Rubelj
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-06
Journal Detail:
Title:  Biogerontology     Volume:  8     ISSN:  1389-5729     ISO Abbreviation:  Biogerontology     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-20     Completed Date:  2007-07-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100930043     Medline TA:  Biogerontology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  163-72     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Ruder Bosković Institute, Bijenicka 54, 10000 Zagreb, Croatia.
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MeSH Terms
Descriptor/Qualifier:
Carbocyanines
Cell Aging*
Cell Proliferation*
Cell Separation / methods
Female
Flow Cytometry
Fluorescent Dyes
Hela Cells
Humans
Phenotype
Telomerase / metabolism*
Time Factors
Tritium
Uterine Cervical Neoplasms / enzymology*,  pathology
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/Carbocyanines; 0/Fluorescent Dyes; 10028-17-8/Tritium; 40957-95-7/3,3'-dioctadecylindocarbocyanine; EC 2.7.7.49/Telomerase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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