| Telomerase activity and hepatic functions of rat embryonic liver progenitor cell in nanoscaffold-coated model bioreactor. | |
| | |
MedLine Citation:
|
PMID: 19816756 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Presently, there is growing interest on telomerase activity in all cells (somatic cells, stem cells, cancerous cells and others) since this activity is associated with cellular changes such as proliferation, differentiation, immortalization, cell injury and ageing. Telomerase activity is absent in most of the somatic cells but present in over 90% of cancerous cells and other immortalized cell lines. In our present study, we cultured a rat embryonal liver progenitor cell line RLC-18 in a self-assembly nanostructured scaffold-coated bioreactor (NCB), collagen-coated plates (CCP) and uncoated plates (UP), and evaluated changes of telomerase activity by non radioactive techniques (Telo TAGGG Telomerase PCR ELISA, cell proliferation based on mitochondria number by MTT assay and hepatic functions such as albumin secretion, urea metabolism, Cytochrome P450 activity like ethoxyresorufin-O-deethylase (EROD) activity. We found less telomerase activity and less cell proliferation, but more hepatic functions on the NCB than on the CCP and UP. Our data support the concept that cell-scaffold interaction may play a significant in controlling the telomerase activity as well as enhanced hepatic functions. Although our present study does not focus on the exact mechanism of telomerase regulation, our result may provide basic clues on cell differentiation whereby telomerase activity inhibits differentiation of cells as in the rat embryonic liver cell line, may be regulated by cell-scaffold interaction and where there is less proliferation, cells perform enhanced hepatic functions, thereby implying that bioartificial liver support may be possible. |
| | |
Authors:
|
Shibashish Giri; Karen Nieber; Ali Acikg?z; Sanja Pavlica; Mario Keller; Augustinus Bader |
Related Documents
:
|
11738146 - Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced express... 11672986 - Mrna levels of the differentiation-associated linker histone variant h1 zero in mitotic... 10853966 - Ultrastructural study on the follicle-associated epithelium of nasal-associated lymphoi... 9380696 - Lack of cell cycle regulation of telomerase activity in human cells. 11834096 - Immunohistochemical analysis of cd1a-labeled langerhans cells in human dental periapica... 21243156 - Hypericin incorporation and localization in fixed hela cells for various conditions of ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-09 |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: 336 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-24 Completed Date: 2010-06-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 137-49 Citation Subset: IM |
Affiliation:
|
Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Deutscher Platz 5, 04103, Leipzig, Germany . |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Albumins
/
secretion Animals Bioreactors* Cell Adhesion Cell Line Cell Proliferation Cell Survival Collagen / chemistry Cytochrome P-450 CYP1A1 / biosynthesis, metabolism Cytochrome P-450 Enzyme System / metabolism Embryonic Stem Cells / cytology, metabolism* Enzyme Induction / drug effects Liver / embryology, metabolism* Nanostructures* / chemistry Peptides / chemistry Rats Surface Properties Telomerase / metabolism* Urea / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Albumins; 0/Peptides; 0/RADA16-I; 57-13-6/Urea; 9007-34-5/Collagen; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Insect chitinase and chitinase-like proteins.
Next Document: Fibronectin-integrin mediated signaling in human cervical cancer cells (SiHa).