Document Detail


Technology insight: in vivo cell tracking by use of MRI.
MedLine Citation:
PMID:  16990841     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Animal studies have shown some success in the use of stem cells of diverse origins to treat heart failure and ventricular dysfunction secondary to ischemic injury. The clinical use of these cells is, therefore, promising. In order to develop effective cell therapies, the location, distribution and long-term viability of these cells must be evaluated in a noninvasive manner. MRI of cells labeled with magnetically visible contrast agents after either direct injection or local or intravenous infusion has the potential to fulfill this goal. In this Review, techniques for labeling and imaging a variety of cells will be discussed. Particular attention will be given to the advantages and limitations of various contrast agents and passive and facilitated cell-labeling methods, as well as to imaging techniques that produce negative and positive contrast, and the effect on image quantification of compartmentalization of contrast agents within the cell.
Authors:
Walter J Rogers; Craig H Meyer; Christopher M Kramer
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Nature clinical practice. Cardiovascular medicine     Volume:  3     ISSN:  1743-4297     ISO Abbreviation:  Nat Clin Pract Cardiovasc Med     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-22     Completed Date:  2006-11-13     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  101226507     Medline TA:  Nat Clin Pract Cardiovasc Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  554-62     Citation Subset:  IM    
Affiliation:
Department of Radiology, University of Virginia Health System, Charlottesville, VA 22908, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Proliferation
Cell Survival
Contrast Media / chemistry,  metabolism*
Dendritic Cells / metabolism
Dextrans
Electroporation / methods
Endocytosis*
Ferumoxytol
Heart Diseases / therapy
Humans
Iron / chemistry,  metabolism
Macrophages / metabolism
Magnetic Resonance Imaging*
Magnetics
Magnetite Nanoparticles
Oxides / chemistry,  metabolism
Receptors, Cell Surface / metabolism
Staining and Labeling* / methods
Stem Cell Transplantation
Stem Cells / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Magnetite Nanoparticles; 0/Oxides; 0/Receptors, Cell Surface; 0/ferumoxtran-10; 1317-61-9/Ferumoxytol; 7439-89-6/Iron; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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