| Technology for cell cycle research with unstressed steady-state cultures. | |
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MedLine Citation:
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PMID: 19002885 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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A culture system for performing cell cycle analyses on cells in undisturbed steady-state populations was designed and tested. In this system, newborn cells are shed continuously from an immobilized, perfused culture rotating about the horizontal axis. As a result of this arrangement, the number of newborn cells released into the effluent medium each generation is identical to the number of cells residing in the immobilized population, indicating that one of the two new daughter cells is shed at each cell division. Thus, the immobilized cells constitute a continuous, steady-state culture because the concentrations, locations and microenvironments of the cells in the culture vessel do not vary with time. In tests with mouse L1210 lymphocytic leukemia cells, about 10(8) newborn cells were produced per day. This new culture system enables a multiplicity of cell cycle analyses on large numbers of cells assured to be from populations in steady-state growth. |
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Authors:
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Valerie S Lebleu; Maureen Thornton; Steven R Gonda; Charles E Helmstetter |
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Publication Detail:
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Type: Journal Article Date: 2006-11-21 |
Journal Detail:
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Title: Cytotechnology Volume: 51 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2008-11-12 Completed Date: 2011-07-14 Revised Date: 2013-03-12 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 149-57 Citation Subset: - |
Affiliation:
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Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL, 32901, USA, valerie_lebleu@hms.harvard.edu. |
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