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Technological properties and probiotic potential of Enterococcus faecium strains isolated from cow milk.
MedLine Citation:
PMID:  23035976     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: To identify enterococci from the fermentation of milk for the production of nono, an African fermented dairy product, to determine the technological properties for suitability as starter cultures and safety as probiotics. METHODS AND RESULTS: Enterococcus faecium CM4 and Enterococcus faecium 2CM1, were isolated from raw cow's milk. The strains were phenotypically and genotypically identified. Technological properties, safety investigations, in vitro adherence properties and antimicrobial characteristics were carried out. Strong acidification and tolerance to bile salts were recorded. The strains were BSH positive and no haemolysis. There was no resistance to clinically relevant antibiotics. The strains exhibited adherence to human collagen type IV, human fibrinogen and fibronectin. The bacteriocins were active against B. cereus DSM 2301, B. subtilis ATCC 6633, Micrococcus luteus and Listeria monocytogenes. Bacteriocins were stable at pH 4-9 and on treatment with lipase, catalase, α-amylase and pepsin, while their activity was lost on treatment with other proteases. The bacteriocins produced were heat stable at 100 °C for 10 min. The bacteriocin produced by the strains was identified as Enterocin A. CONCLUSIONS: The Enterococcus faecium strains in this study exhibited probiotic activity and the safety investigations indicate their suitability as good candidates for a starter culture fermentation process. SIGNIFICANCE AND IMPACT OF STUDY: The use of bacteriocin producing Enterococcus faecium strains as starter cultures in fermented foods is beneficial but however, their safety investigations as probiotics must be greatly emphasized. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for AppliedMicrobiology.
Authors:
Kolawole Banwo; Abiodun Sanni; Huarong Tan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Department of Microbiology, University of Ibadan, P.M.B 1 Ibadan, Oyo State, Nigeria; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
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