| Techniques for cryopreservation of individual or small numbers of human spermatozoa: a systematic review. | |
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MedLine Citation:
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PMID: 19109313 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreservation of individual or small numbers of human spermatozoa in patients with severe male factor of infertility. METHODS: We searched the MEDLINE, EMBASE, Cochrane Systematic Reviews, CENTRAL, Web of Science, Scopus databases for relevant studies up to June of 2008. The search used terms referring to cryopreservation of small amount of sperm. Included studies were limited to human studies with no language restrictions. RESULTS: We identified 30 reports including 9 carriers used for cryopreservation of small quantities/numbers of human spermatozoa (7 non-biological and 2 biological carriers). A wide variety of cryopreservation vehicles were reported. The recovery rate of spermatozoa cryopreserved in a known small number varied widely from 59 to 100%. Fertilization rates were in the range of 18-67%. Frozen-thawed spermatozoa, using this method, were subsequently used for intracytoplasmic sperm injection in only five studies, with few pregnancies reported so far. To date, there remains no consensus as to the ideal carrier for cryopreservation of small number of spermatozoa for clinical purposes. CONCLUSIONS: Cryopreservation of individual or small numbers of human spermatozoa may replace the need for repeated surgical sperm retrieval. A controlled multicenter trial with sufficient follow-up would provide valid evidence of the potential benefit of this approach. |
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Authors:
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Faten AbdelHafez; Mohamed Bedaiwy; Sherif A El-Nashar; Edmund Sabanegh; Nina Desai |
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Publication Detail:
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Type: Journal Article; Review Date: 2008-12-24 |
Journal Detail:
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Title: Human reproduction update Volume: 15 ISSN: 1460-2369 ISO Abbreviation: Hum. Reprod. Update Publication Date: 2009 Mar-Apr |
Date Detail:
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Created Date: 2009-02-05 Completed Date: 2009-04-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9507614 Medline TA: Hum Reprod Update Country: England |
Other Details:
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Languages: eng Pagination: 153-64 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Cleveland Clinic Fertility Centre, Cleveland Clinic Foundation, OH 44122, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cohort Studies Cryopreservation / methods* Cryoprotective Agents Female Humans Male Pregnancy Pregnancy Outcome Reproductive Techniques, Assisted Sperm Retrieval Spermatozoa* |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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