| Technique and staining optimization leucoconcentration. | |
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MedLine Citation:
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PMID: 2444399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In cytometric clinical application, it is important to obtain cell suspensions rapidly with as little cytological alteration as possible. A procedure has been achieved to prepare cell suspensions for flow cytometric analysis. The leucoconcentration technique, first described by Herbeuval for cytologic analysis, has been modified to be applied in cytometry. This technique involves Saponin lysis of red cells of peripheral blood or bone marrow samples that have been previously fixed with picric acid alcohol solution. Cells in suspension are not shifted and tinctorial affinity is not modified. Then cells have been stained with Mithramycin. Each parameter defined by Crissman has been analyzed to define the best staining conditions. The availability of Leucoconcentration with Mithramycin-DNA-staining permits determination of cell cycle with a fine resolution. |
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Authors:
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J Pierrez; A Guerci; O Guerci |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytometry Volume: 8 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1987 Sep |
Date Detail:
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Created Date: 1987-12-14 Completed Date: 1987-12-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 529-33 Citation Subset: IM |
Affiliation:
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Laboratoire de Cytométrie en Flux, CHU Nancy-Brabois, 54500 Vandoeuvre les Nancy, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bone Marrow Cells Cell Separation / methods* DNA / analysis* Erythrocytes Humans Hydrogen-Ion Concentration Leukocytes / analysis, cytology* Magnesium Magnesium Chloride Osmolar Concentration Plicamycin Staining and Labeling* Time Factors |
| Chemical | |
Reg. No./Substance:
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18378-89-7/Plicamycin; 7439-95-4/Magnesium; 7786-30-3/Magnesium Chloride; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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