Document Detail


Technique for continuous measurement of compliance in isolated vascular segments.
MedLine Citation:
PMID:  3942248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method has been developed that provides an on-line (1/s) measurement of elastance [E = 1/compliance (C)] in a perfused isolated vessel segment in situ. Values of E can be obtained as a function of perfusion pressure (P) or in response to drug interventions. Noncompliant stainless steel tubes are used as inflow and outflow cannulas. Segment P is obtained from a short small-bore stainless steel catheter and miniature pressure transducer. During constant flow through the inflow cannula, an adjustable outflow resistance is used to set the segment P. Simultaneous activation of solenoid pinch valves, for 200 ms, compresses in-line Silastic tubing next to each cannula and serves to simultaneously 1) isolate the segment and 2) inject a small volume into the segment (delta V). This delta V results in a step increase in segment P (delta P). delta V is not significantly altered by changing the volume of air in the in-line windkessel reservoir (when air volume is greater than or equal to 100 ml), mean perfusion pressure (over a range of 50-150 mmHg) or compliance of the test cell. Therefore, delta P alpha E, and 1/delta P alpha C. Since this recorded variable is linearly related to C, actual C can be continuously recorded on a recorder after a simple two-point calibration process. C as determined by this method was found to be linearly related (r = 0.997) to calculated C of a test cell air bubble (range: 0.1 less than C less than 0.7 microliter/mmHg; resolution: 0.001 microliter/mmHg).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
L B Bell; E J Zuperku; J P Kampine
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  250     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-02-20     Completed Date:  1986-02-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R142-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Calibration
Carotid Sinus / physiology
Computers
Dogs
Evaluation Studies as Topic
Physiology / instrumentation,  methods*
Vascular Resistance*
Grant Support
ID/Acronym/Agency:
GM-29641/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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