Document Detail

A Technique for Precise Inoculation of the Internal Phyllosphere of Cotton with Xanthomonas campestris pv. malvacearum.
MedLine Citation:
PMID:  18944317     Owner:  NLM     Status:  In-Data-Review    
ABSTRACT A technique was developed to inoculate uniformly and gently the internal phyllosphere from the upper surface of cotton leaves with the phytopathogenic bacterium Xanthomonas campestris pv. malvacearum. The inoculum consisted of 2 to 3 x 10(7) CFU/ml in CaCO(3)-saturated sterile distilled water containing 0.02%, vol/vol, of the wetting agent Silwet L-77. A custom-made inoculation apparatus was employed to immerse a circular area of the adaxial surface of a leaf in inoculum for 90 s. This resulted in uniform, passive entry of bacteria into the substomatal chambers, producing an endophytic bacterial population of 2 x 10(4) CFU/cm(2). Microscopic signs of infection were visible 48 to 72 h after inoculation. In susceptible leaves, uniformly distributed water-soaked spots were observed 7 to 8 days after inoculation. When the technique was used on resistant leaves, the autofluorescence that is characteristic of hypersensitively necrotic cells developed in the guard cells and palisade cells lining substomatal chambers, but not in the underlying spongy mesophyll.
N Kangatharalingam; Margaret L Pierce; Margaret Essenberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytopathology     Volume:  93     ISSN:  0031-949X     ISO Abbreviation:  Phytopathology     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2008-10-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9427222     Medline TA:  Phytopathology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1204-8     Citation Subset:  -    
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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