Document Detail


Technical-scale production of cyanophycin with recombinant strains of Escherichia coli.
MedLine Citation:
PMID:  12089018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By the use of Escherichia coli DH1 harboring cphA from Synechocystis sp. strain PCC6803, large-scale production of cyanophycin at 30- and 500-liter culture volumes was established. Transcription of cphA was controlled by the thermosensitive cI857 repressor, which enabled induction of cphA by a simple temperature shift in the culture fluid. Maximum cyanophycin cell content of up to 24% (wt/wt) of cellular dry matter was obtained by induction in the early exponential growth phase and cultivation of the cells in terrific broth complex medium. Synthesis of cyanophycin was found to be strongly dependent on the presence of complex components, and in mineral salts medium the cells synthesized and accumulated cyanophycin only if Casamino Acids were added. Cultivations were done at the 500-liter scale, allowing the provision of cell mass for the preparation of cyanophycin at the kilogram scale. Isolation of cyanophycin was achieved by a new acid extraction procedure which allowed large-scale purification of the polyamide from whole cells.
Authors:
Kay M Frey; Fred B Oppermann-Sanio; Holger Schmidt; Alexander Steinbüchel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-28     Completed Date:  2002-07-29     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3377-84     Citation Subset:  IM    
Affiliation:
Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Corrensstrasse 3, D-48149 Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins*
Culture Media
Cyanobacteria / genetics
Escherichia coli / genetics,  metabolism*
Plant Proteins / metabolism*
Recombination, Genetic
Transformation, Bacterial
beta-Lactamases / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/Plant Proteins; 0/cyanophycin; EC 3.5.2.6/beta-Lactamases
Comments/Corrections

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