Document Detail

Technical and physiological aspects associated with the lower fertilization following intra cytoplasmic sperm injection (ICSI) in human.
MedLine Citation:
PMID:  10732119     Owner:  NLM     Status:  MEDLINE    
The fertilization rates with ICSI range from 30% to 70% and suggest that, despite injecting sperm into mature oocytes, significant fertilization failure still occurs in humans. The objective of this study was to determine technical and physiological factors which may contribute to lower fertilization following ICSI. Eggs that failed to show two pronuclei (PN) 48 hours after ICSI were studied at two different time intervals: at ICSI program inception (group A) and after 8 months (group B). The eggs were analyzed by staining with DNA fluorochromes, Hoescht 33258 and DAPI. The extent of sperm head as well as maternal chromatin decondensation in unfertilized ICSI eggs was determined by high resolution fluorescence microscopy. The average fertilization rate (FR) from all ICSI cycles in these two groups was 45%. The FR in Groups A and B were 35% and 59%, respectively (P < 0.05). In Group A, 65% of the unfertilized eggs were characterized by condensed sperm chromatin with 11% showing partial decondensation. In Group B, only 28% of the unfertilized eggs demonstrated condensed sperm chromatin while 45% were partially decondensed. Sperm chromatin was not detected in 24% of all unfertilized eggs studied. The maternal chromatin remained at metaphase II in 84% of all unfertilized eggs analyzed. These observations suggest that the technical problem of deposition of the sperm inside the egg is not the major cause for failure of fertilization rates in ICSI cycles. The increased percentage of eggs undergoing sperm head decondensation may be related to subtle changes in technique as experience is gained over time. The failure of sperm head decondensation in some of the ICSI eggs may be associated with cytoplasmic immaturity but not nuclear maturity.
A K Dubey; A S Penzias; R H Reindollar; T Ducibella
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  49     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1998 Jan 
Date Detail:
Created Date:  2000-04-07     Completed Date:  2000-04-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  33-41     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Tufts University School of Medicine, Boston, MA, USA.
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MeSH Terms
Chromatin / ultrastructure
Oocytes / cytology*
Sperm Head / ultrastructure
Sperm Injections, Intracytoplasmic*
Spermatozoa / cytology*
Treatment Failure
Treatment Outcome
Reg. No./Substance:

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