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Technical note: Stearidonic acid metabolism by mixed ruminal microorganisms in vitro.
MedLine Citation:
PMID:  22021809     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Dietary supplementation of stearidonic acid (SDA; 18:4n-3) has been considered to be a possible strategy to increase n-3 unsaturated fatty acid content in ruminant products, however little is known about its metabolism in the rumen. In vitro batch incubations were carried out with bovine ruminal digesta to investigate the metabolism of SDA and its biohydrogenation products. Incubation mixtures (4.5 mL) contained 0 (SDA0, control), 0.25 (SDA0.25), 0.50 (SDA0.5), 0.75 (SDA0.75), 1.00 (SDA1), 1.25 (SDA1.25), and 1.50 (SDA1.5) mg SDA supplemented to 33 mg (DM basis) commercial total mixed ration (TMR) based on corn silage, for dairy cows, were incubated for 72 h at 39 °C. The content of most fatty acids in whole freeze-dried cultures was affected by SDA supplementation. Branched-chain fatty acids decreased linearly (P < 0.01), and odd-chain fatty acids decreased quadratically (P < 0.01), particularly from SDA1 and above, whereas most C18 fatty acids increased linearly or quadratically (P ≤ 0.04). Stearidonic acid concentrations at 72 h were very low, < 0.6% of total fatty acids and ≤ 0.9% of added SDA, in all treatments. The apparent biohydrogenation of SDA was extensive, but it was not affected by SDA concentration (P > 0.05). Biohydrogenation followed a pattern similar to other C18 unsaturated fatty acids up to SDA1, stearic acid (18:0) and vaccenic acid (18:1 trans-11) being the major fatty acids formed, the latter increasing 9 fold in SDA1. At higher inclusion rates, 18:0 and 18:1 trans isomers decreased (P ≤ 0.03), accompanied by increases in unidentified 18:3 and 18:4 isomers (P = 0.02), suggesting that the biohydrogenation pathway was inhibited. The present results indicate clearly that SDA was metabolized extensively, with numerous 18:4 and 18:3 products formed en route to further conversion to 18:2, 18:1 isomers and 18:0.
Authors:
M R G Maia; C A S Correia; S P Alves; A J M Fonseca; A R J Cabrita
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-21
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
REQUIMTE, ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão VC, Portugal.
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