Document Detail


Technical note: Rapid method for determination of amino acids in milk.
MedLine Citation:
PMID:  20494144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid method for measurement of amino acids in milk was developed and validated. The method included a first step of milk protein hydrolysis, followed by the derivatization and separation of amino acids by HPLC. Six combinations of hydrolysis agent and temperature-time conditions were compared with a reference method; derivatization procedures as well as HPLC separation were improved. Hydrolysis of milk samples with 6 N HCl at 160 degrees C for 60 min resulted in no significantly differences compared with the reference method but allowed the analysis of a greater number of milk samples in a short time. In addition, this method was characterized by high precision, low repeatability uncertainty, and high accuracy for all amino acids evaluated; the recovery mean value of the single amino acids was 98.38%. The proposed method is, therefore, accurate, simple, rapid, and suitable for large numbers of milk samples.
Authors:
R Marino; M Iammarino; A Santillo; M Muscarella; M Caroprese; M Albenzio
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  93     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2367-70     Citation Subset:  IM    
Copyright Information:
2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Production and Innovation in Mediterranean Agriculture and Food System (PrIME) University of Foggia, Via Napoli, 25-71100 Foggia, Italy. r.marino@unifg.it
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Animals
Cattle
Chromatography, High Pressure Liquid
Hydrolysis
Milk / chemistry*
Milk Proteins / chemistry
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Milk Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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