| Technical note: Rapid method for determination of amino acids in milk. | |
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MedLine Citation:
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PMID: 20494144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid method for measurement of amino acids in milk was developed and validated. The method included a first step of milk protein hydrolysis, followed by the derivatization and separation of amino acids by HPLC. Six combinations of hydrolysis agent and temperature-time conditions were compared with a reference method; derivatization procedures as well as HPLC separation were improved. Hydrolysis of milk samples with 6 N HCl at 160 degrees C for 60 min resulted in no significantly differences compared with the reference method but allowed the analysis of a greater number of milk samples in a short time. In addition, this method was characterized by high precision, low repeatability uncertainty, and high accuracy for all amino acids evaluated; the recovery mean value of the single amino acids was 98.38%. The proposed method is, therefore, accurate, simple, rapid, and suitable for large numbers of milk samples. |
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Authors:
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R Marino; M Iammarino; A Santillo; M Muscarella; M Caroprese; M Albenzio |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of dairy science Volume: 93 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2367-70 Citation Subset: IM |
Copyright Information:
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2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Production and Innovation in Mediterranean Agriculture and Food System (PrIME) University of Foggia, Via Napoli, 25-71100 Foggia, Italy. r.marino@unifg.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Animals Cattle Chromatography, High Pressure Liquid Hydrolysis Milk / chemistry* Milk Proteins / chemistry Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Milk Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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