Document Detail

Technical note: Improved extraction method with hexane for gas chromatographic analysis of conjugated linoleic acids.
MedLine Citation:
PMID:  16357271     Owner:  NLM     Status:  MEDLINE    
Extraction properties of different solvents (chloroform/methanol, hexane/isopropanol, and hexane) were studied for the gas chromatographic analysis of conjugated linoleic acids (CLA) from probiotic bacteria grown in de Man, Rogosa, and Sharpe medium. As compared with chloroform/methanol and hexane/isopropanol, hexane showed comparable extraction efficiency for CLA from unspent de Man, Rogosa, and Sharpe medium, but showed minimal extraction of oleic acid originated from the emulsifier in broth. The extraction efficiency of CLA by hexane was influenced by the broth pH, showing the optimal pH of 7.0. Repeated extraction with hexane increased the yield. Extraction with hexane showed excellent recovery of spiked CLA from the spent broth with up to 97.2% (standard deviation of 1.74%). This represents the highest recovery of CLA from culture broth ever reported. The sample size was also successfully reduced to 0.5 mL to analyze CLA from the broth without impairment of analytical data. This smaller sample size in the 1.5-mL microcentrifuge tube using a small bench-top centrifuge reduced analytical time significantly.
M Y Jung; G-B Kim; E S Jang; Y K Jung; S Y Park; B H Lee
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  89     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-16     Completed Date:  2006-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  90-4     Citation Subset:  IM    
Department of Food Science and Agricultural Chemistry, McGill University, Ste-Anne de-Bellevue, QC, H9X 3V9, Canada.
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MeSH Terms
Bifidobacterium / growth & development,  metabolism
Chromatography, Gas / methods*
Culture Media
Hydrogen-Ion Concentration
Linoleic Acids, Conjugated / isolation & purification*
Probiotics / metabolism
Reg. No./Substance:
0/Culture Media; 0/Hexanes; 0/Linoleic Acids, Conjugated; 0/Solvents; 67-56-1/Methanol; 67-63-0/2-Propanol; 67-66-3/Chloroform

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