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Technical note: Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism.
MedLine Citation:
PMID:  22999279     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We report the development of a PCR-single strand conformation polymorphism (SSCP) method to identify Prototheca spp. responsible for bovine mastitis: P. zopfii and P. blaschkeae. The method was set up using reference strains belonging to P. zopfii genotype 1, P. zopfii genotype 2, and P. blaschkeae as target species and P. stagnora, and P. ulmea as negative controls. The assay was applied on 50 isolates of Prototheca spp. isolated from bovine mastitic milk or bulk-tank milk samples, and all isolates were identified as P. zopfii genotype 2. We conclude that the described PCR-SSCP approach is accurate, inexpensive, and highly suitable for the identification of P. zopfii genotype 2 on field isolates but also directly on milk, if preceded by a specific DNA extraction method.
Authors:
P Cremonesi; F Pozzi; M Ricchi; B Castiglioni; M Luini; S Chessa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-19
Journal Detail:
Title:  Journal of dairy science     Volume:  -     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Affiliation:
Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche (CNR), via Einstein, 26900 Lodi, Italy. Electronic address: cremonesi@ibba.cnr.it.
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