Document Detail


Technical improvement of the TBP (tubulin-based polymorphism) method for plant species detection, based on capillary electrophoresis.
MedLine Citation:
PMID:  23019101     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Nowadays, feed and food safety and traceability are of primary importance. Hence, a correct labeling of the different products is highly desirable in general, but mandatory for those people who are suffering from eating disorders and food allergies. Among the technologies that have been developed for feed and food analysis, the patented tubulin-based polymorphism (TBP) method emerges as an easy, versatile, and inexpensive diagnostic tool. Initially used to fingerprint different plant species and varieties, TBP was then successfully applied to trace species in mixtures of plant origin such as commercial feeds. TBP is a DNA-based molecular marker, that makes use of PCR for the selective amplification of plant β-tubulin introns. Amplified fragments are then separated by PAGE and visualized by silver staining. We have now developed an improved version of TBP. Based on capillary electrophoresis and fluorescence detection, it makes the method automatic, more sensible, reproducible, and faster. Compared to the classic TBP, this new version allows to obtain a better data resolution and an easier interpretation of the results, clearing the way to large-scale feed/food diagnostics.
Authors:
Floriana Gavazzi; Anna Paola Casazza; Claudia Depedro; Francesco Mastromauro; Diego Breviario
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  33     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2840-51     Citation Subset:  IM    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Istituto di Biologia e Biotecnologia Agraria - IBBA, Consiglio Nazionale delle Ricerche, Milan, Italy.
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