| Technical Advance: The rat aorta contains resident mononuclear phagocytes with proliferative capacity and proangiogenic properties. | |
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MedLine Citation:
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PMID: 20628067 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Angiogenesis in the aortic ring model is preceded by activation of the immune system and impaired by ablation of adventitial macrophages. Treatment of aortic cultures with M-CSF induced extensive periaortic outgrowth of CD45(+) CD68(+) mononuclear cells with ultrastructural features of macrophages and DCs. Periaortic lysis of collagen caused many CD45(+) CD68(+) cells to attach to the bottom of the culture dish. Lifting the collagen gels left behind patches of CD45(+) CD68(+) cells, which focally organized into branching cords. These cells also expressed CD14, CD169, F4/80, and α-SMA but not CD31, vWF, desmin, or CD163. DNA synthesis studies showed that M-CSF-stimulated cells were actively proliferating. Aortic patch cells showed phagocytic properties and responded to IL-4 and GM-CSF by expressing MHC II, differentiating into DCs, and forming multinucleated giant cells. They also stimulated angiogenesis and VEGF production in aortic ring cultures. This study demonstrates that the rat aorta contains a distinct subset of immature immunocytes capable of proliferating, differentiating into macrophages and DCs, and stimulating angiogenesis. Isolation of these cells in patches from M-CSF-stimulated aortic rings provides a reproducible system to study the biology and angiogenic role of the resident immune system of the aortic wall. |
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Authors:
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Penelope Zorzi; Alfred C Aplin; Kelly D Smith; Roberto F Nicosia |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-07-13 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 88 ISSN: 1938-3673 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-02 Completed Date: 2010-11-24 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1051-9 Citation Subset: IM |
Affiliation:
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VA Puget Sound Health Care System, 1660 South Columbian Way, Seattle, WA 98108, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Antigens, CD / genetics, immunology Antigens, CD45 / immunology Antigens, Differentiation, Myelomonocytic / immunology Aorta / cytology*, drug effects, immunology, ultrastructure Cell Division DNA Primers Immunoenzyme Techniques Macrophage Colony-Stimulating Factor / pharmacology Microscopy, Confocal / methods Neovascularization, Physiologic Phagocytes / cytology*, drug effects Phagocytosis Rats Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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HL-52585/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 protein, rat; 0/DNA Primers; 81627-83-0/Macrophage Colony-Stimulating Factor; EC 3.1.3.48/Antigens, CD45 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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