| Technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium-201 uptake in rat myocardial cells. | |
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MedLine Citation:
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PMID: 9476934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanisms of uptake and intracellular distribution of 99mTc-tetrofosmin, 99mTc-MIBI and 201TI and the behaviors of 99mTc-tetrofosmin and 99mTc-MIBI in relation to Na+ were studied with primary cultures of myocardial cells. METHODS: Both the uptake and the washout of the tracers were sequentially measured. The cells were treated with ouabain, bumetanide, tetrodotoxin, dimethyl amiloride (DMA), nigericin and carbonyl cyanide m-chlorophenylhydrazone (CCCP) to observe the effects of the uptake and intracellular distribution of the traders. Cells equilibrated in buffers with or without Na+ were treated with monensin and DMA to evaluate the effect of Na+ on the accumulation of the tracers. RESULTS: Despite the similarities in uptake kinetics, there was a higher level of retention of 99mTc-tetrofosmin inside the cells. Ouabain, bumetanide and tetrodotoxin did not show any inhibitory effect on the uptake of 99mTc-tetrofosmin and 99mTc-MIBI, whereas they produced various degrees of inhibition of 201TI uptake. DMA produced approximately 35% inhibition of 99mTc-tetrofosmin uptake and 50% inhibition of 99mTc-MIBI uptake. Nigericin increased the uptake of 99mTc-MIBI by the cells. The addition of CCCP produced the release of 38% of the accumulated 99mTc-tetrofosmin and 52%-70% of the accumulated 99mTc-MIBI, indicating that these percentages of accumulation were related to mitochondrial uptake. Neither Na+-free buffer nor monensin had any significant effect on 99mTc-tetrofosmin accumulation, but they both caused increased accumulation of 99mTc-MIBI. CONCLUSION: The uptake of both 99mTc-tetrofosmin and 99mTc-MIBI through the cell membrane is partly related to the Na+/H+ antiporter system. Only part of the accumulated 99mTc-tetrofosmin inside the cells enters into mitochondria; most of the accumulated 99mc-MIBI is related to mitochondrial uptake. This uptake may be related to Na+. |
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Authors:
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A S Arbab; K Koizumi; K Toyama; T Arai; T Araki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of nuclear medicine : official publication, Society of Nuclear Medicine Volume: 39 ISSN: 0161-5505 ISO Abbreviation: J. Nucl. Med. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-03-12 Completed Date: 1998-03-12 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217410 Medline TA: J Nucl Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 266-71 Citation Subset: IM |
Affiliation:
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Department of Radiology, Yamanashi Medical University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amiloride
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analogs & derivatives,
pharmacology Animals Bumetanide / pharmacology Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology Cell Survival Cells, Cultured Heart / radionuclide imaging* Ion Transport / drug effects Membrane Potentials Mitochondria, Heart / physiology Monensin / pharmacology Myocardium / cytology, metabolism Nigericin / pharmacology Organophosphorus Compounds / diagnostic use*, pharmacokinetics Organotechnetium Compounds / diagnostic use*, pharmacokinetics Ouabain / pharmacology Radiopharmaceuticals / diagnostic use*, pharmacokinetics Rats Rats, Sprague-Dawley Sodium / metabolism Technetium Tc 99m Sestamibi / diagnostic use*, pharmacokinetics Tetrodotoxin / pharmacology Thallium Radioisotopes / diagnostic use*, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Organophosphorus Compounds; 0/Organotechnetium Compounds; 0/Radiopharmaceuticals; 0/Thallium Radioisotopes; 0/technetium Tc 99m 1,2-bis(bis(2-ethoxyethyl)phosphino)ethane; 109581-73-9/Technetium Tc 99m Sestamibi; 1214-79-5/5-dimethylamiloride; 17090-79-8/Monensin; 2609-46-3/Amiloride; 28380-24-7/Nigericin; 28395-03-1/Bumetanide; 4368-28-9/Tetrodotoxin; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 630-60-4/Ouabain; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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