| Technetium-99m direct radiolabeling of lanreotide: a somatostatin analog. | |
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MedLine Citation:
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PMID: 11573798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lanreotide, a synthetic octapeptide analog of a native hormone somatostatin, was labeled with a commonly available, inexpensive radionuclide 99mTc. Labeling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelation with 99mTc. Stannous chloride was used as reducing agent, while tartrate acted as transchelating agent. Lanreotide (100 microg), stannous chloride dihydrate (100 microg) and tartaric acid (64 microg) were dissolved in acetate/acetic acid buffer (pH 2.8). After overnight (approximately 18 h) incubation, approximately 444 MBq (12 mCi) 99mTc was added and kept in boiling water for 30 min. More than 97% labeling efficiency was confirmed by RP-HPLC, ITLC-SG and C18 cartridge analysis. Radiolabeling results in one major peak when analyzed by reverse-phase HPLC. The stability of the 99mTc-peptide bond was evaluated by cysteine challenge studies. |
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Authors:
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S Pervez; A M Mushtaq; M Arif |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine Volume: 55 ISSN: 0969-8043 ISO Abbreviation: Appl Radiat Isot Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-09-27 Completed Date: 2001-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306253 Medline TA: Appl Radiat Isot Country: England |
Other Details:
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Languages: eng Pagination: 647-51 Citation Subset: IM |
Affiliation:
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Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, Nilore, Islamabad, Pakistan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Cysteine Humans Peptides, Cyclic / chemical synthesis*, diagnostic use, isolation & purification Radiopharmaceuticals / chemical synthesis*, diagnostic use, isolation & purification Somatostatin / analogs & derivatives, chemical synthesis*, diagnostic use, isolation & purification Technetium / diagnostic use, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Peptides, Cyclic; 0/Radiopharmaceuticals; 118992-92-0/lanreotide; 51110-01-1/Somatostatin; 52-90-4/Cysteine; 7440-26-8/Technetium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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