Document Detail

Technetium-99m direct radiolabeling of lanreotide: a somatostatin analog.
MedLine Citation:
PMID:  11573798     Owner:  NLM     Status:  MEDLINE    
Lanreotide, a synthetic octapeptide analog of a native hormone somatostatin, was labeled with a commonly available, inexpensive radionuclide 99mTc. Labeling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelation with 99mTc. Stannous chloride was used as reducing agent, while tartrate acted as transchelating agent. Lanreotide (100 microg), stannous chloride dihydrate (100 microg) and tartaric acid (64 microg) were dissolved in acetate/acetic acid buffer (pH 2.8). After overnight (approximately 18 h) incubation, approximately 444 MBq (12 mCi) 99mTc was added and kept in boiling water for 30 min. More than 97% labeling efficiency was confirmed by RP-HPLC, ITLC-SG and C18 cartridge analysis. Radiolabeling results in one major peak when analyzed by reverse-phase HPLC. The stability of the 99mTc-peptide bond was evaluated by cysteine challenge studies.
S Pervez; A M Mushtaq; M Arif
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine     Volume:  55     ISSN:  0969-8043     ISO Abbreviation:  Appl Radiat Isot     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-09-27     Completed Date:  2001-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306253     Medline TA:  Appl Radiat Isot     Country:  England    
Other Details:
Languages:  eng     Pagination:  647-51     Citation Subset:  IM    
Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, Nilore, Islamabad, Pakistan.
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MeSH Terms
Chromatography, High Pressure Liquid
Peptides, Cyclic / chemical synthesis*,  diagnostic use,  isolation & purification
Radiopharmaceuticals / chemical synthesis*,  diagnostic use,  isolation & purification
Somatostatin / analogs & derivatives,  chemical synthesis*,  diagnostic use,  isolation & purification
Technetium / diagnostic use,  isolation & purification
Reg. No./Substance:
0/Peptides, Cyclic; 0/Radiopharmaceuticals; 118992-92-0/lanreotide; 51110-01-1/Somatostatin; 52-90-4/Cysteine; 7440-26-8/Technetium

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