Document Detail


Teaching Single-Cell Digital Analysis Using Droplet-Based Microfluidics.
MedLine Citation:
PMID:  22229495     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Microfluidics allows the manipulation of small quantities of reagents in a high-throughput manner and is therefore highly amenable to single cell characterization and more generally to digital analysis, with applications in fields as varied as genomics, diagnostics, directed evolution, and drug screening. The growing place of microfluidics in biology laboratories encouraged us to develop a teaching method where advanced undergraduate or first-year graduate-level students are taught to fabricate droplet-based microfluidic devices, characterize them, and finally use them to perform a digital analysis of bacterial samples based on a phenotypic marker.
Authors:
Majdi Najah; Andrew D Griffiths; Michael Ryckelynck
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-9
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg , CNRS UMR 7006, 8 allée Gaspard Monge, 67083 Strasbourg Cedex, France.
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